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Literature summary for 1.7.3.3 extracted from

  • Khade, S.; Srivastava, S.; Kumar, K.; Sharma, K.; Goyal, A.; Tripathi, A.
    Optimization of clinical uricase production by Bacillus cereus under submerged fermentation, its purification and structure characterization (2018), Process Biochem., 75, 49-58 .
No PubMed abstract available
Search for more literature for 1.7.3.3

Application

Application Comment Organism
medicine the enzyme can be used for treating gout disease Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Bacillus cereus
-
 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O Bacillus cereus MTCC 8550
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus MTCC 8550
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 75 gel filtration Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.7
-
 crude extract, at pH 7.5 and 30°C Bacillus cereus
87
-
 after 11.3fold purification, at pH 7.5 and 30°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Bacillus cereus 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O
-
 Bacillus cereus MTCC 8550 5-hydroxyisourate + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Bacillus cereus
? x * 34400, calculated from amino acid sequence Bacillus cereus

Synonyms

Synonyms Comment Organism
uricase
-
 Bacillus cereus

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus cereus partially purified enzyme, isoelectric focusing
-
 5