Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Candida sp. (in: Saccharomycetales) |
Protein Variants | Comment | Organism |
---|---|---|
C249S | the substitution has no effect on enzymatic activity | Candida sp. (in: Saccharomycetales) |
C249S/DELTAL302 | the combination of both mutations increases the enzymatic activity by 8%.Tthe specific activity of the mutant enzyme does not decrease after exposed to 37°C for 20 h | Candida sp. (in: Saccharomycetales) |
DELTAL302 | the mutant has improved specific activity (6%) compared with wild type enzyme | Candida sp. (in: Saccharomycetales) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Candida sp. (in: Saccharomycetales) | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Candida sp. (in: Saccharomycetales) | - |
5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida sp. (in: Saccharomycetales) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography and Superdex 200 gel filtration | Candida sp. (in: Saccharomycetales) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Candida sp. (in: Saccharomycetales) | 5-hydroxyisourate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
uricase | - |
Candida sp. (in: Saccharomycetales) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the specific activity of wild type enzyme decreases to 90% after exposed to 37°C for 20h | Candida sp. (in: Saccharomycetales) |