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Literature summary for 1.7.3.5 extracted from

  • Porter, D.J.T.; Bright, H.J.
    Propionate-3-nitronate oxidase from Penicillium atrovenetum is a flavoprotein which initiates the autoxidation of its substrate by O2 (1987), J. Biol. Chem., 262, 14428-14434.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
propane-2-nitronate increases the enzyme activity over 40fold when added to a reaction mixture that contains propionate-3-nitronate, the stimulation can be partially inhibited by superoxide dismutase Penicillium atrovenetum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Penicillium atrovenetum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE in presence of mercaptoethanol Penicillium atrovenetum
73000
-
gel filtration Penicillium atrovenetum

Organism

Organism UniProt Comment Textmining
Penicillium atrovenetum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Penicillium atrovenetum

Reaction

Reaction Comment Organism Reaction ID
3-aci-nitropropanoate + O2 + H2O = 3-oxopropanoate + nitrite + H2O2 primary products of the enzymatic reaction probably is the nitropropanoate free radical and superoxide Penicillium atrovenetum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Penicillium atrovenetum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
160
-
butyrate-4-nitronate as substrate Penicillium atrovenetum
274
-
propionate-3-nitronate as substrate Penicillium atrovenetum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aci-nitropropanoate + O2
-
Penicillium atrovenetum 3-oxopropanoate + nitrite + H2O2
-
?
butyrate-4-nitronate + O2
-
Penicillium atrovenetum succinate + nitrite + H2O2
-
?
additional information neither cytochrome c nor K3Fe(CN)6 serve as oxidants, other substrates tested are oxidized less than 2% the rate of 3-nitropropanoate Penicillium atrovenetum ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE in presence of mercaptoethanol Penicillium atrovenetum

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Penicillium atrovenetum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
350
-
3-aci-Nitropropanoate
-
Penicillium atrovenetum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
assay at Penicillium atrovenetum

Cofactor

Cofactor Comment Organism Structure
FMN flavoprotein Penicillium atrovenetum