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Literature summary for 1.7.3.6 extracted from

  • Wehrfritz, J.; Carter, J.; Spiro, S.; Richardson, D.
    Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species (1996), Arch. Microbiol., 166, 421-424.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
horse heart ferricytochrome c in 10 mM Tris-HC1 (pH 8.0), at 30°C Pseudomonas sp.
0.35
-
hydroxylamine in 10 mM Tris-HC1 (pH 8.0), at 30°C Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 19000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. S2.14
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
in 10 mM Tris-HC1 (pH 8.0), at 30°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + horse heart ferricytochrome c
-
Pseudomonas sp. nitroxyl + horse heart ferrocytochrome c + H+
-
?
hydroxylamine + horse heart ferricytochrome c
-
Pseudomonas sp. S2.14 nitroxyl + horse heart ferrocytochrome c + H+
-
?
additional information the enzyme utilizes horse-heart cytochrome c as an electron acceptor but is unable to donate electrons to purified Pseudomonas denitrificans cytochrome c550 or pseudoazurin, or to Pseudomonas aeruginosa azurin Pseudomonas sp. ?
-
?
additional information the enzyme utilizes horse-heart cytochrome c as an electron acceptor but is unable to donate electrons to purified Pseudomonas denitrificans cytochrome c550 or pseudoazurin, or to Pseudomonas aeruginosa azurin Pseudomonas sp. S2.14 ?
-
?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
HAO
-
Pseudomonas sp.
hydroxylamine-cytochrome c oxidoreductase
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
heme
-
Pseudomonas sp.