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Literature summary for 1.7.5.2 extracted from

  • de Vries, S.; Strampraad, M.J.; Lu, S.; Moenne-Loccoz, P.; Schroder, I.
    Purification and characterization of the MQH2:NO oxidoreductase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2003), J. Biol. Chem., 278, 35861-35868.
    View publication on PubMed
Search for more literature for 1.7.5.2

Inhibitors

Inhibitors Comment Organism Structure
NO substrate inhibition at high concentratins Pyrobaculum aerophilum

Localization

Localization Comment Organism GeneOntology No. Textmining
integral to membrane
-
 Pyrobaculum aerophilum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron qNOR contains two heme iron and one nonheme iron Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78800
-
 1 * 78800, deduced from nucleotide sequence Pyrobaculum aerophilum
127000
-
 gel filtration Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NO + menaquinol Pyrobaculum aerophilum
-
 N2O + H2O + menaquinone
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1% dodecyl maltoside, CM-Sepharose, hydroxyapatite Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.28
-
 at 54.5°C Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NO + menaquinol
-
 Pyrobaculum aerophilum N2O + H2O + menaquinone
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000-80000, SDS-PAGE, assuming that qNOR binds one dodecyl maltoside micelle of about 40000-50000 Da Pyrobaculum aerophilum
monomer 1 * 78800, deduced from nucleotide sequence Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
qNOR
-
 Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
93
-
 qNOR in membranes Pyrobaculum aerophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 93 no activity detectable below 25°C Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 no loss of activity after 24 h, qNOR in membrane Pyrobaculum aerophilum
56
-
 50% loss of activity after 630 min, qNOR in membrane Pyrobaculum aerophilum
75
-
 50% loss of activity after 231 min, qNOR in membrane Pyrobaculum aerophilum
100
-
 50% loss of activity after 4 min, purified qNOR Pyrobaculum aerophilum
100
-
 50% loss of activity after 86 min, qNOR in membrane Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Pyrobaculum aerophilum

pH Range

pH Minimum pH Maximum Comment Organism
4 10 approx. 50% of maximal activity at pH 5.0, almost no activity at pH 10.0 Pyrobaculum aerophilum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 NO 54.5°C, pH 7.0, qNOR in membrane Pyrobaculum aerophilum
0.015
-
 NO 54.5°C, pH 7.0, purified qNOR Pyrobaculum aerophilum