Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cyanidioschyzon merolae |
Protein Variants | Comment | Organism |
---|---|---|
G212S/L213T/Y214L/S217C/C220I/S221N | mutations mimic partially isoform SiRA | Cyanidioschyzon merolae |
S217C | mutation mimics the corresponding residue in isoform SiRA, recovers sulfite reduction activity | Cyanidioschyzon merolae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
nitrite | mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
0.237 | - |
nitrite | truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
0.395 | - |
nitrite | truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyanidioschyzon merolae | M1URK1 | cf. EC 1.8.7.1, isoform SiRA | - |
Cyanidioschyzon merolae | M1V771 | cf. EC 1.8.7.1, isoform SiRB | - |
Cyanidioschyzon merolae 10D | M1V771 | cf. EC 1.8.7.1, isoform SiRB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme additionally shows activity of sulfite reductase, EC 1.8.7.1 | Cyanidioschyzon merolae | ? | - |
? | |
additional information | enzyme additionally shows activity of sulfite reductase, EC 1.8.7.1 | Cyanidioschyzon merolae 10D | ? | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | - |
Cyanidioschyzon merolae | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? | |
nitrite + 6 reduced ferredoxin + 7 H+ | - |
Cyanidioschyzon merolae 10D | NH3 + 2 H2O + 6 oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYME_CMG021C | - |
Cyanidioschyzon merolae |
CYME_CMJ117C | - |
Cyanidioschyzon merolae |
SiRA | - |
Cyanidioschyzon merolae |
SirB | - |
Cyanidioschyzon merolae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.77 | - |
nitrite | mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
2.1 | - |
nitrite | truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae | |
3.77 | - |
nitrite | truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication | Cyanidioschyzon merolae |
Organism | Comment | Expression |
---|---|---|
Cyanidioschyzon merolae | enzyme is constitutively expressed | additional information |