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Literature summary for 1.7.7.1 extracted from

  • Sekine, K.; Moriyama, T.; Kim, J.; Hase, T.; Sato, N.
    Characterization of two ferredoxin-dependent sulfite reductases having different substrate specificity in the red alga Cyanidioschyzon merolae (2017), J. Biochem., 162, 37-43 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cyanidioschyzon merolae

Protein Variants

Protein Variants Comment Organism
G212S/L213T/Y214L/S217C/C220I/S221N mutations mimic partially isoform SiRA Cyanidioschyzon merolae
S217C mutation mimics the corresponding residue in isoform SiRA, recovers sulfite reduction activity Cyanidioschyzon merolae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
nitrite mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
0.237
-
nitrite truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
0.395
-
nitrite truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae

Organism

Organism UniProt Comment Textmining
Cyanidioschyzon merolae M1URK1 cf. EC 1.8.7.1, isoform SiRA
-
Cyanidioschyzon merolae M1V771 cf. EC 1.8.7.1, isoform SiRB
-
Cyanidioschyzon merolae 10D M1V771 cf. EC 1.8.7.1, isoform SiRB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme additionally shows activity of sulfite reductase, EC 1.8.7.1 Cyanidioschyzon merolae ?
-
?
additional information enzyme additionally shows activity of sulfite reductase, EC 1.8.7.1 Cyanidioschyzon merolae 10D ?
-
?
nitrite + 6 reduced ferredoxin + 7 H+
-
Cyanidioschyzon merolae NH3 + 2 H2O + 6 oxidized ferredoxin
-
?
nitrite + 6 reduced ferredoxin + 7 H+
-
Cyanidioschyzon merolae 10D NH3 + 2 H2O + 6 oxidized ferredoxin
-
?

Synonyms

Synonyms Comment Organism
CYME_CMG021C
-
Cyanidioschyzon merolae
CYME_CMJ117C
-
Cyanidioschyzon merolae
SiRA
-
Cyanidioschyzon merolae
SirB
-
Cyanidioschyzon merolae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.77
-
nitrite mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
2.1
-
nitrite truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
3.77
-
nitrite truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae

Expression

Organism Comment Expression
Cyanidioschyzon merolae enzyme is constitutively expressed additional information