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Literature summary for 1.7.7.2 extracted from

  • Rubio, L.M.; Herrero, A.; Flores, E.
    A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase (1996), Plant Mol. Biol., 30, 845-850.
    View publication on PubMed
Search for more literature for 1.7.7.2

Cloned(Commentary)

Cloned (Comment) Organism
narB structural gene for nitrate reductase is cloned and expressed in Escherichia coli as a 76 kDa polypeptide with 729 amino acids Synechococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7 2.1 nitrate
-
 Synechococcus sp.
1.5
-
 nitrate reduced methyl viologen as electron donor, recombinant enzyme expressed in Escherichia coli Synechococcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant enzyme activity is found exclusively in the soluble fraction Synechococcus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + reduced ferredoxin Synechococcus sp. assimilatory nitrate reduction nitrite + oxidized ferredoxin
-
 ?
nitrate + reduced ferredoxin Synechococcus sp. reduced ferredoxin as physiological electron donor nitrite + oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
 strain PCC 7942
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + reduced ferredoxin ratio of methyl viologen- to ferredoxin-dependent nitrate reduction is about 27 Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
 ?
nitrate + reduced ferredoxin assimilatory nitrate reduction Synechococcus sp. nitrite + oxidized ferredoxin
-
 ?
nitrate + reduced ferredoxin reduced ferredoxin as physiological electron donor Synechococcus sp. nitrite + oxidized ferredoxin
-
 ?
nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Synechococcus sp. nitrite + oxidized methyl viologen
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, enzyme expressed in Escherichia coli, SDS-PAGE Synechococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 reduced methyl viologen as electron donor Synechococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10.5 reduced methyl viologen as electron donor Synechococcus sp.

Cofactor

Cofactor Comment Organism Structure
additional information narB gene with domains that might be involved in binding of the molybdenum cofactor and of an iron-sulfur cluster, but no flavin nucleotide- or heme-binding domains Synechococcus sp.