Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidinium chloride | 25% inhibition at 2 mM, 30% inhibition at 3 mM | Cucurbita pepo |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
hydroxylamine reductase 2, gel filtration | Cucurbita pepo |
60000 | - |
hydroxylamine reductase 1, gel filtration | Cucurbita pepo |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita pepo | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cucurbita pepo |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Cucurbita pepo | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activities after different purification methods | Cucurbita pepo |
Storage Stability | Organism |
---|---|
-18°C, DEAE-cellulose fraction, often stable for several weeks, but unpredictable loss of activity | Cucurbita pepo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + reduced acceptor | hydroxylamine reductase 1 reacts with reduced benzyl viologen, ferredoxin and FMNH2 | Cucurbita pepo | ammonia + oxidized acceptor | - |
? | |
hydroxylamine + reduced acceptor | hydroxylamine reductase 2 reacts with benzyl viologen and dithionite | Cucurbita pepo | ammonia + oxidized acceptor | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, 25-40% loss of activity | Cucurbita pepo |
60 | - |
10 min, 50-85% loss of activity | Cucurbita pepo |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no clear or consistent pH optima observed with hydroxylamine reductase 1 and hydroxylamine reductase 2 | Cucurbita pepo |