Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
thio-NADPH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.008 | - |
beta-NADPH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.023 | - |
reduced beta-nicotinamide hypoxanthine dinucleotide phosphate | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.043 | - |
NADH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.055 | - |
alpha-NADPH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.073 | - |
mycothione | - |
Mycobacterium tuberculosis | |
0.09 | - |
thio-NADH | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.2 | - |
reduced beta-nicotinamide hypoxanthine dinucleotide | des-myo-inositol mycothione as second substrate | Mycobacterium tuberculosis | |
0.24 | - |
2-methyl-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
0.34 | - |
5,8-Dihydroxy-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
0.51 | - |
des-myo-inositol mycothione | - |
Mycobacterium tuberculosis | |
0.54 | - |
5-hydroxy-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
3.3 | - |
DTNB | - |
Mycobacterium tuberculosis | |
4 | - |
2,6-dimethylbenzoquinone | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
alpha2, 2 * 50000, SDS-PAGE | Mycobacterium tuberculosis |
100000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHH3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHH3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.63 | - |
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethylbenzoquinone + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
2,6-dimethylbenzoquinone + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
2-methyl-1,4-naphthoquinone + alpha-NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
5,8-dihydroxy-1,4-naphthoquinone + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
5-hydroxy-1,4-naphthoquinone + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
5-hydroxy-1,4-naphthoquinone + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
des-myo-inositol mycothione + NADH | - |
Mycobacterium tuberculosis | ? | - |
? | |
des-myo-inositol mycothione + reduced beta-nicotinamide hypoxanthine dinucleotide | - |
Mycobacterium tuberculosis | ? | - |
? | |
des-myo-inositol mycothione + reduced beta-nicotinamide hypoxanthine dinucleotide phosphate | - |
Mycobacterium tuberculosis | ? | - |
? | |
des-myo-inositol mycothione + thio-NADH | - |
Mycobacterium tuberculosis | ? | - |
? | |
des-myo-inositol mycothione + thio-NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
desmyoinositol mycothione + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
desmyoinositol mycothione + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
DTNB + NADPH | - |
Mycobacterium tuberculosis | ? | - |
? | |
DTNB + NADPH | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
mycothione + NADPH | - |
Mycobacterium tuberculosis | mycothiol + NADP+ | - |
? | |
mycothione + NADPH | - |
Mycobacterium tuberculosis H37Rv | mycothiol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | alpha2, 2 * 50000, SDS-PAGE | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per monomer | Mycobacterium tuberculosis | |
NADPH | - |
Mycobacterium tuberculosis |