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Literature summary for 1.8.1.15 extracted from
- Identification of a TeO32- reductase/mycothione reductase from Rhodococcus erythropolis PR4 (2020), FEMS Microbiol. Ecol., 97, fiaa220 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhodococcus erythropolis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.413 | - |
TeO32- | in PBS at pH 7.4, temperature not specified in the publication | Rhodococcus erythropolis | |
| 0.517 | - |
TeO32- | in 20 mM HEPES, at pH 7.0, temperature not specified in the publication | Rhodococcus erythropolis | |
| 0.779 | - |
TeO32- | in 20 mM TEA-HCl, at pH 7.6, temperature not specified in the publication | Rhodococcus erythropolis | |
| 35.23 | - |
SeO32- | in 20 mM TEA-HCl, at pH 7.6, temperature not specified in the publication | Rhodococcus erythropolis |  |
| 41.14 | - |
SeO32- | in 20 mM HEPES, at pH 7.0, temperature not specified in the publication | Rhodococcus erythropolis | |
| 43.9 | - |
SeO32- | in 20 PBS, at pH 7.4, temperature not specified in the publication | Rhodococcus erythropolis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mycothione + NADPH + H+ | Rhodococcus erythropolis | - |
2 mycothiol + NADP+ | - |
? | |
| mycothione + NADPH + H+ | Rhodococcus erythropolis PR4 | - |
2 mycothiol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
- |
- |
| Rhodococcus erythropolis PR4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and HiTrap DEAE column chromatography | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mycothione + NADPH + H+ | - |
Rhodococcus erythropolis | 2 mycothiol + NADP+ | - |
? | |
| mycothione + NADPH + H+ | - |
Rhodococcus erythropolis PR4 | 2 mycothiol + NADP+ | - |
? | |
| SeO32- + NADPH + H+ | the enzyme exibits a strong substrate preference for TeO32- over SeO32- | Rhodococcus erythropolis | ? | - |
? | |
| SeO32- + NADPH + H+ | the enzyme exibits a strong substrate preference for TeO32- over SeO32- | Rhodococcus erythropolis PR4 | ? | - |
? | |
| TeO32- + NADPH + H+ | the enzyme exibits a strong substrate preference for TeO32- over SeO32- | Rhodococcus erythropolis | ? | - |
? | |
| TeO32- + NADPH + H+ | the enzyme exibits a strong substrate preference for TeO32- over SeO32- | Rhodococcus erythropolis PR4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MTR | - |
Rhodococcus erythropolis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
TeO32- | in PBS at pH 7.4, temperature not specified in the publication | Rhodococcus erythropolis | |
| 1 | - |
TeO32- | in 20 mM HEPES, at pH 7.0, temperature not specified in the publication | Rhodococcus erythropolis | |
| 1.48 | - |
TeO32- | in 20 mM TEA-HCl, at pH 7.6, temperature not specified in the publication | Rhodococcus erythropolis | |
| 3.67 | - |
SeO32- | in 20 PBS, at pH 7.4, temperature not specified in the publication | Rhodococcus erythropolis | |
| 5.46 | - |
SeO32- | in 20 mM HEPES, at pH 7.0, temperature not specified in the publication | Rhodococcus erythropolis | |
| 8.29 | - |
SeO32- | in 20 mM TEA-HCl, at pH 7.6, temperature not specified in the publication | Rhodococcus erythropolis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodococcus erythropolis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
SeO32- | in 20 mM HEPES, at pH 7.0, temperature not specified in the publication | Rhodococcus erythropolis | |
| 0.24 | - |
SeO32- | in 20 mM TEA-HCl, at pH 7.6, temperature not specified in the publication | Rhodococcus erythropolis | |
| 0.48 | - |
TeO32- | in PBS at pH 7.4, temperature not specified in the publication | Rhodococcus erythropolis | |
| 1.9 | - |
TeO32- | in 20 mM TEA-HCl, at pH 7.6, temperature not specified in the publication | Rhodococcus erythropolis | |
| 1.93 | - |
TeO32- | in 20 mM HEPES, at pH 7.0, temperature not specified in the publication | Rhodococcus erythropolis | |
| 11.96 | - |
SeO32- | in 20 PBS, at pH 7.4, temperature not specified in the publication | Rhodococcus erythropolis | |
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