Cloned (Comment) | Organism |
---|---|
expressed in LMG194 Escherichia coli cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F496D | variants of the flavin binding reductase (SiRFP) shows reduced activity compared to that of the wild-type enzyme | Escherichia coli |
V500D | variant of the flavin binding reductase (SiRFP) binds NADP+ with an affinity similar to that of wild-type enzyme. The variant is reduced by about 60% in its ability to transfer electrons to the iron-containing oxidase (SiRHP). | Escherichia coli |
Y101A | variants of the flavin binding reductase (SiRFP) shows reduced activity compared to that of the wild-type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + 3 NADPH + 3 H+ | Escherichia coli | essential enzyme in the global sulfur cycle | hydrogen sulfide + 3 NADP+ + 3 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + 3 NADPH + 3 H+ | - |
Escherichia coli | hydrogen sulfide + 3 NADP+ + 3 H2O | - |
? | |
sulfite + 3 NADPH + 3 H+ | essential enzyme in the global sulfur cycle | Escherichia coli | hydrogen sulfide + 3 NADP+ + 3 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | NADPH-dependent assimilatory sulfite reductase (SiR) is composed of two subunits. One is a multidomain flavin binding reductase (SiRFP) and the other an iron-containing oxidase (SiRHP). Tight binding between SiRFP and SiRHP is incompatible with the electron transfer-competent interface, suggesting that the dodecameric holoenzyme consists of four SiRFP/SiRHP heterodimers and four free SiRFP molecules. Six electrons come two at a time from three NADPH cofactors to complete catalysis, which means that SiRHP must interact with SiRFP three times for every SO3(2-) that binds | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent assimilatory sulfite reductase | - |
Escherichia coli |
SIR | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | essential enzyme in the global sulfur cycle | Escherichia coli |