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Literature summary for 1.8.1.4 extracted from

  • Igamberdiev, A.U.; Bykova, N.V.; Ens, W.; Hill, R.D.
    Dihydrolipoamide dehydrogenase from porcine heart catalyzes NADH-dependent scavenging of nitric oxide (2004), FEBS Lett., 568, 146-150.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methylene blue stimulates reduction of cytochrome c and myoglobin Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of the nitric oxide reduction by cyanide, antimycin A, or diphenyleneiodonium ions Sus scrofa
N-ethylmaleimide over 90% inhibition at 1 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
NO nitric oxide reduction, pH 7.5 Sus scrofa
0.01
-
NADH nitric oxide reduction, pH 7.5 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa the enzyme might play a role in modifying NO levels under specific cell conditions ?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
further purification of the commercial preparation Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Sus scrofa
-
heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa
0.5
-
metmyoglobin reduction, in absence of methylene blue, pH 7.5 Sus scrofa
0.6
-
O2 reduction, pH 6.0 Sus scrofa
0.8
-
cytochrome c reduction, in absence of methylene blue, pH 7.5 Sus scrofa
1.7
-
lipoic acid reduction, pH 6.0 Sus scrofa
4.5
-
metmyoglobin reduction, in presence of methylene blue, pH 7.5 Sus scrofa
6.4
-
cytochrome c reduction, in presence of methylene blue, pH 7.5 Sus scrofa
7.2
-
2,6-dichlorophenolindophenol reduction, pH 6.0 Sus scrofa
154
-
lipoamide reduction, pH 6.0 Sus scrofa
210
-
nitric oxide reduction, pH 6.0 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADH cytochrome c is a poor electron acceptor, only in presence of methylene blue the enzyme shows some activity Sus scrofa 2 ferrocytochrome c + NAD+ + H+
-
?
metmyoglobin + NADH myoglobin is a poor electron acceptor, only in presence of methylene blue the enzyme shows some activity Sus scrofa reduced myoglobin + NAD+
-
?
additional information the enzyme might play a role in modifying NO levels under specific cell conditions Sus scrofa ?
-
?
NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Sus scrofa NAD+ + reduced 2,6-dichlorophenolindophenol
-
?
nitric oxide + NADH
-
Sus scrofa nitrate + NAD+
-
?
O2 + NADH
-
Sus scrofa H2O2 + NAD+
-
?
oxidized lipoamide + NADH
-
Sus scrofa reduced lipoamide + NAD+
-
?
oxidized lipoic acid + NADH
-
Sus scrofa reduced lipoic acid + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
Sus scrofa
DLDH
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 reduction of nitric oxide, lioamide, lipoic acid, and 2,6-dichlorophenolindophenol Sus scrofa
7.5
-
reduction of cytochrome c and myoglobin, oxidation of NADH Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5 8 nitric oxide reduction Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sus scrofa
additional information no activity with NADPH Sus scrofa
NADH
-
Sus scrofa