Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.4 extracted from

  • Kim, H.
    Asparagine-473 residue is important to the efficient function of human dihydrolipoamide dehydrogenase (2005), J. Biochem. Mol. Biol., 38, 248-252.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of D473L mutant in Escherichia coli strain XL1-Blue Homo sapiens

Protein Variants

Protein Variants Comment Organism
D473L site-directed mutagenesis, mutant shows about 37fold decreased activity and small conformational changes compared to the wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrolipoamide + NAD+ Homo sapiens
-
lipoamide + NADH
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant D473L mutant from Escherichia coli to homogeneity Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ Asp473 is important for efficient catalytic function of the enzyme Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.6
-
purified recombinant mutant D473L Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
Homo sapiens lipoamide + NADH
-
?

Subunits

Subunits Comment Organism
More the homodimeric enzyme is the E3 component of 2-ketoacid dehydrogenase multienzyme complex Homo sapiens

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
Homo sapiens
dihydrolipoamide:NAD+ oxidoreductase
-
Homo sapiens
E3
-
Homo sapiens
More enzyme is the E3 component of 2-ketoacid dehydrogenase multienzyme complex Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group, 1 FAD per enzyme subunit Homo sapiens
NAD+
-
Homo sapiens