Cloned (Comment) | Organism |
---|---|
expression of D473L mutant in Escherichia coli strain XL1-Blue | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D473L | site-directed mutagenesis, mutant shows about 37fold decreased activity and small conformational changes compared to the wild-type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | Homo sapiens | - |
lipoamide + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant D473L mutant from Escherichia coli to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | Asp473 is important for efficient catalytic function of the enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.6 | - |
purified recombinant mutant D473L | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the homodimeric enzyme is the E3 component of 2-ketoacid dehydrogenase multienzyme complex | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
More | enzyme is the E3 component of 2-ketoacid dehydrogenase multienzyme complex | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | prosthetic group, 1 FAD per enzyme subunit | Homo sapiens | |
NAD+ | - |
Homo sapiens |