Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D444V | missense mutation, expressed at 28ºC the mutant exhibits essentially wild-type E3 activity, at 37°C the activity is reduced to 12% of that of the wild type enzyme | Homo sapiens |
E340K | missense mutation, expressed at 28ºC the mutant exhibits essentially wild-type E3 activity, at 37°C the activity is reduced to 38% of that of the wild type enzyme | Homo sapiens |
R447G | missense mutation, expressed at 28ºC the mutant exhibits essentially wild-type E3 activity, at 37°C the activity is reduced to 28% of that of the wild type enzyme | Homo sapiens |
R460G | missense mutation, the dissociation constant is three orders of magnitude higher than that of wild-type E3 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S-100 column gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NAD+ | - |
Homo sapiens |