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Literature summary for 1.8.1.4 extracted from
- The role of amino acids T148 and R281 in human dihydrolipoamide dehydrogenase (2008), J. Biomed. Sci., 15, 37-46.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | T148 is not important to E3 catalytic function, whereas R281 plays a role in the catalytic function of E3 | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutants cloned into pQE-9 vector | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R281K | specific activity is 11.93% to that of wild-type E3. FAD-content is about 93% that of wild-type E3. Kcat of forward reaction is decreased dramatically. Substitution has no effect in the self-dimerization | Homo sapiens |
| R281N | specific activity is 12.50% to that of wild-type E3. FAD-content is about 96% that of wild-type E3. Kcat of forward reaction is decreased dramatically. Substitution has no effect in the self-dimerization | Homo sapiens |
| T148G | specific activity is 76.34% to that of wild-type E3. FAD-content is about 710% that of wild-type E3. Substitution has no effect in the self-dimerization | Homo sapiens |
| T148S | specific activity is 88.62% to that of wild-type E3. FAD-content is about 92% that of wild-type E3. Substitution has no effect in the self-dimerization | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
dihydrolipoamide | mutant R281K | Homo sapiens |  |
| 0.22 | - |
NAD+ | wild-type E3 | Homo sapiens | |
| 0.28 | - |
NAD+ | mutant T148G | Homo sapiens | |
| 0.29 | - |
NAD+ | mutant T148S | Homo sapiens | |
| 0.38 | - |
dihydrolipoamide | mutant R281N | Homo sapiens | |
| 0.38 | - |
dihydrolipoamide | wild-type E3 | Homo sapiens | |
| 0.43 | - |
NAD+ | mutant R281K | Homo sapiens | |
| 0.5 | - |
NAD+ | mutant R281N | Homo sapiens | |
| 0.58 | - |
dihydrolipoamide | mutant T148G | Homo sapiens | |
| 0.59 | - |
dihydrolipoamide | mutant T148S | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
molecular sieving HPLC | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P09622 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged E3 wild-type and mutant proteins | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | molecular sieving HPLC | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrolipoamide dehydrogenase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 220 | - |
dihydrolipoamide | mutant R281N | Homo sapiens | |
| 223 | - |
dihydrolipoamide | mutant R281K | Homo sapiens | |
| 505 | - |
dihydrolipoamide | wild-type E3 | Homo sapiens | |
| 655 | - |
dihydrolipoamide | mutant T148G | Homo sapiens | |
| 742 | - |
dihydrolipoamide | mutant T148S | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
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