Cloned (Comment) | Organism |
---|---|
gene lpd2, recombinant expression of GUS-linked enzyme in Arabidopsis thaliana ecotype Col-0 in cotyledons, rosette leaves and roots (in the cap of the established lateral roots, but not in the cap of the main roots) via transformation by Agrobacterium tumefaciens strain GV3101 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of DLDH2 enzyme knockout mutant plants, the aos phenotype in mtlpd2-2 is due to disruption of mtLPD2. Mutation of mtLPD2 enhances As(V)-induced changes in metabolite pools, aos phenotype analysis, overview | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | activity of lipoamide dehydrogenase in isolated mitochondria is sensitive to arsenite, but not arsenate | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | Arabidopsis thaliana | - |
lipoamide + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M5K2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | mtLPD2 has a tissue-dependent expression pattern | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Arabidopsis thaliana | lipoamide + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, about, sequence calculation, x * 55000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
DLDH2 | - |
Arabidopsis thaliana |
lipoamide dehydrogenase | - |
Arabidopsis thaliana |
lipoamide dehydrogenase2 | - |
Arabidopsis thaliana |
LPD2 | - |
Arabidopsis thaliana |
mtLPD2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | metabolic acclimation of Arabidopsis thaliana to arsenate is sensitized by the loss of mitochondrial lipoamide dehydrogenase2. Both arsenate and arsenite inhibit root elongation, decreased seedling size and increase anthocyanin production more profoundly in knockout mutants than in wild-type seedlings, arsenite seems to be the mediator of the observed phenotypes | Arabidopsis thaliana |
metabolism | the enzyme is a key enzyme in oxidative metabolism | Arabidopsis thaliana |
additional information | mitochondrial lipoamide dehydrogenase is an important protein for determining the sensitivity of oxidative metabolism to arsenate in Arabidopsis thaliana | Arabidopsis thaliana |