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Literature summary for 1.8.1.4 extracted from

  • Chen, W.; Taylor, N.L.; Chi, Y.; Millar, A.H.; Lambers, H.; Finnegan, P.M.
    The metabolic acclimation of Arabidopsis thaliana to arsenate is sensitized by the loss of mitochondrial lipoamide dehydrogenase2, a key enzyme in oxidative metabolism (2014), Plant Cell Environ., 37, 684-695 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lpd2, recombinant expression of GUS-linked enzyme in Arabidopsis thaliana ecotype Col-0 in cotyledons, rosette leaves and roots (in the cap of the established lateral roots, but not in the cap of the main roots) via transformation by Agrobacterium tumefaciens strain GV3101 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of DLDH2 enzyme knockout mutant plants, the aos phenotype in mtlpd2-2 is due to disruption of mtLPD2. Mutation of mtLPD2 enhances As(V)-induced changes in metabolite pools, aos phenotype analysis, overview Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
arsenite activity of lipoamide dehydrogenase in isolated mitochondria is sensitive to arsenite, but not arsenate Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrolipoamide + NAD+ Arabidopsis thaliana
-
lipoamide + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M5K2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information mtLPD2 has a tissue-dependent expression pattern Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
Arabidopsis thaliana lipoamide + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 54000, about, sequence calculation, x * 55000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
DLDH2
-
Arabidopsis thaliana
lipoamide dehydrogenase
-
Arabidopsis thaliana
lipoamide dehydrogenase2
-
Arabidopsis thaliana
LPD2
-
Arabidopsis thaliana
mtLPD2
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction metabolic acclimation of Arabidopsis thaliana to arsenate is sensitized by the loss of mitochondrial lipoamide dehydrogenase2. Both arsenate and arsenite inhibit root elongation, decreased seedling size and increase anthocyanin production more profoundly in knockout mutants than in wild-type seedlings, arsenite seems to be the mediator of the observed phenotypes Arabidopsis thaliana
metabolism the enzyme is a key enzyme in oxidative metabolism Arabidopsis thaliana
additional information mitochondrial lipoamide dehydrogenase is an important protein for determining the sensitivity of oxidative metabolism to arsenate in Arabidopsis thaliana Arabidopsis thaliana