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Literature summary for 1.8.1.4 extracted from
- Characterization of two site-specific mutations in human dihydrolipoamide dehydrogenase deteriorating the apparent enzyme binding affinities to both dihydrolipoamide and NAD+ (2018), J. Korean Chem. Soc., 62, 253-256 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P154A | the mutation makes enzyme binding to both dihydrolipoamide and NAD+ inefficient | Homo sapiens |
| P423A | the mutation makes enzyme binding to both dihydrolipoamide and NAD+ inefficient | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |  |
| 0.27 | - |
NAD+ | mutant enzyme P154A, at pH 8.0 and 37°C | Homo sapiens | |
| 0.4 | - |
NAD+ | mutant enzyme P423A, at pH 8.0 and 37°C | Homo sapiens | |
| 0.64 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
| 0.7 | - |
dihydrolipoamide | mutant enzyme P423A, at pH 8.0 and 37°C | Homo sapiens | |
| 0.87 | - |
dihydrolipoamide | mutant enzyme P154A, at pH 8.0 and 37°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P09622 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA His-Bind resin column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrolipoamide dehydrogenase | - |
Homo sapiens |
| dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
| E3 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 711 | - |
dihydrolipoamide | mutant enzyme P423A, at pH 8.0 and 37°C | Homo sapiens | |
| 899 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
| 1309 | - |
dihydrolipoamide | mutant enzyme P154A, at pH 8.0 and 37°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| NAD+ | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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