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Literature summary for 1.8.1.4 extracted from

  • Kim, H.
    Characterization of a human dihydrolipoamide dehydrogenase mutant showing significantly decreased catalytic efficiency toward NAD+ (2020), J. Korean Chem. Soc., 64, 141-144 .
No PubMed abstract available
Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
P355A the mutation makes enzyme binding to NAD+ substantially less efficient. The catalytic efficiency of the mutant toward NAD+ is decreased by 81% compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens
0.52
-
 dihydrolipoamide mutant enzyme P355A, at pH 8.0 and 37°C Homo sapiens
0.64
-
 dihydrolipoamide wild type enzyme, at pH 8.0 and 37°C Homo sapiens
0.7
-
 NAD+ mutant enzyme P355A, at pH 8.0 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09622
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
 Homo sapiens lipoamide + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Homo sapiens
homodimer 2 * 50216, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
 Homo sapiens
dihydrolipoamide:NAD+ oxidoreductase
-
 Homo sapiens
E3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
624
-
 NAD+ mutant enzyme P355A, at pH 8.0 and 37°C Homo sapiens
624
-
 dihydrolipoamide mutant enzyme P355A, at pH 8.0 and 37°C Homo sapiens
899
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens
899
-
 dihydrolipoamide wild type enzyme, at pH 8.0 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
NAD+
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
891
-
 NAD+ mutant enzyme P355A, at pH 8.0 and 37°C Homo sapiens
1200
-
 dihydrolipoamide mutant enzyme P355A, at pH 8.0 and 37°C Homo sapiens
1405
-
 dihydrolipoamide wild type enzyme, at pH 8.0 and 37°C Homo sapiens
4732
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens