Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | no inhibition | Homo sapiens | |
glutathione | product inhibition | Homo sapiens | |
N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
NADP+ | - |
Homo sapiens | |
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Saccharomyces cerevisiae | |
NADPH | - |
Homo sapiens | |
p-chloromercuriphenyl sulfonate | - |
Saccharomyces cerevisiae | |
phenyl mercuric acetate | - |
Saccharomyces cerevisiae | |
phosphate buffer | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
NADPH | - |
Saccharomyces cerevisiae | |
0.0095 | - |
NADPH | 0.03 M phosphate buffer | Homo sapiens | |
0.013 | - |
NADPH | 0.3 M phosphate buffer | Homo sapiens | |
0.019 | - |
GSSG | 0.03 M phosphate buffer | Homo sapiens | |
0.055 | - |
GSSG | - |
Saccharomyces cerevisiae | |
0.125 | - |
GSSG | 0.3 M phosphate buffer | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | cytoplasm-like spaces of mitochondria | Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | Homo sapiens | - |
glutathione + NADP+ | - |
? | |
GSSG + NADPH | Saccharomyces cerevisiae | - |
glutathione + NADP+ | - |
? | |
GSSG + NADPH | Hemicentrotus pulcherrimus | - |
glutathione + NADP+ | - |
? | |
GSSG + NADPH | Escherichia coli | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | Rattus norvegicus | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | Rattus norvegicus | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
ir | |
GSSG + NADPH | Penicillium chrysogenum | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | Escherichia coli B / ATCC 11303 | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | Escherichia coli Crookes | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | Escherichia coli M191-6 | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
review | - |
Hemicentrotus pulcherrimus | - |
sea urchin, review | - |
Homo sapiens | - |
- |
- |
Penicillium chrysogenum | - |
review | - |
Rattus norvegicus | - |
review | - |
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
? | |
GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
r | |
GSSG + NADPH | - |
Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
GSSG + NADPH | - |
Hemicentrotus pulcherrimus | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli | glutathione + NADP+ | - |
r | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Rattus norvegicus | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Rattus norvegicus | glutathione + NADP+ | - |
r | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Saccharomyces cerevisiae | glutathione + NADP+ | - |
r | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Penicillium chrysogenum | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Penicillium chrysogenum | glutathione + NADP+ | - |
r | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Hemicentrotus pulcherrimus | glutathione + NADP+ | - |
? | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli | glutathione + NADP+ | - |
? | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Rattus norvegicus | glutathione + NADP+ | - |
? | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Rattus norvegicus | glutathione + NADP+ | - |
ir | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Penicillium chrysogenum | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli B / ATCC 11303 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli B / ATCC 11303 | glutathione + NADP+ | - |
r | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli B / ATCC 11303 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli Crookes | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli Crookes | glutathione + NADP+ | - |
r | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli Crookes | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli M191-6 | glutathione + NADP+ | - |
? | |
GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli M191-6 | glutathione + NADP+ | - |
r | |
GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli M191-6 | glutathione + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Homo sapiens |
25 | - |
assay at | Saccharomyces cerevisiae |
25 | - |
assay at | Hemicentrotus pulcherrimus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.3 | - |
glutathione disulfide | reoxidation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
broad optimum | Homo sapiens |
7 | - |
NADPH + GSSG | Rattus norvegicus |
7 | - |
NADPH + GSSG | Saccharomyces cerevisiae |
8 | - |
glutathione disulfide oxidation | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
FAD | - |
Rattus norvegicus | |
FAD | - |
Penicillium chrysogenum | |
FAD | - |
Hemicentrotus pulcherrimus | |
FAD | 5 thiol groups per FAD | Escherichia coli | |
FAD | 4 thiol groups per FAD | Saccharomyces cerevisiae | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Saccharomyces cerevisiae | |
NADPH | - |
Penicillium chrysogenum | |
NADPH | - |
Hemicentrotus pulcherrimus |