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Literature summary for 1.8.1.7 extracted from
- Glutathione reductase from Rhodospirillum rubrum (1969), Arch. Mikrobiol., 66, 374-388.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Br- | - |
Rhodospirillum rubrum |  |
| citrate | - |
Rhodospirillum rubrum | |
| Cl- | - |
Rhodospirillum rubrum | |
| glutathione | GSSG, 12 mM, 25% inhibition | Rhodospirillum rubrum | |
| N-ethylmaleimide | - |
Rhodospirillum rubrum | |
| NO3- | - |
Rhodospirillum rubrum | |
| p-chloromercuribenzoate | - |
Rhodospirillum rubrum | |
| SO42- | - |
Rhodospirillum rubrum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
NADPH | - |
Rhodospirillum rubrum | |
| 0.058 | - |
GSSG | - |
Rhodospirillum rubrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
gel filtration | Rhodospirillum rubrum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodospirillum rubrum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodospirillum rubrum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
purified enzyme | Rhodospirillum rubrum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 6 days | Rhodospirillum rubrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Rhodospirillum rubrum | glutathione + NADP+ | - |
? | |
| additional information | bisubstrate mechanism | Rhodospirillum rubrum | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
24 h, stable | Rhodospirillum rubrum |
| 55 | - |
5 min, no loss of activity | Rhodospirillum rubrum |
| 60 | - |
3 min, 30% loss of activity | Rhodospirillum rubrum |
| 62 | - |
unstable above | Rhodospirillum rubrum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.2 | - |
Rhodospirillum rubrum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD enzyme | Rhodospirillum rubrum | |
| NADH | 3% activity of that with NADPH | Rhodospirillum rubrum | |
| NADPH | - |
Rhodospirillum rubrum | |
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Release 2023.1 (January 2023)
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