Crystallization (Comment) | Organism |
---|---|
structure analysis overview, ligand binding, active center | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ajoene | i.e. (E,Z)-4,5,9-trithiadodeca-1,6,11-triene-9-oxide, natural compound from garlic, Allium sativum, covalent inhibition, but also substrate; time- and temperature-dependent inhibition, mixed disulfide between cative site Cys58 and the inhibitor, modified enzyme shows a markedly increased oxidative activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
diallyl trisulfide | - |
Homo sapiens | |
0.38 | - |
(Z)-ajoene | - |
Homo sapiens | |
0.8 | - |
(E)-ajoene | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Z)-ajoene + NADPH + H+ | - |
Homo sapiens | 4,5,9-trithiadodeca-1,6,11-triene + 4,8,9,13-tetrathiahexadeca-1,6,10,15-tetraene + NADP+ | - |
r | |
2 ajoene + 4 NADPH + 4 H+ | i.e. (E,Z)-4,5,9-trithiadodeca-1,6,11-triene-9-oxide, substrate and inhibitor | Homo sapiens | 4,8,9,13-tetrathiahexadeca-1,6,1,15-tetraene + 4 NADP+ + 2 allylmercaptan | - |
? | |
2 ajoene + 4 NADPH + 4 H+ | also formation of single-electron reduced product and concomitantly superoxide anion radicals | Homo sapiens | 4,8,9,13-tetrathiahexadeca-1,6,1,15-tetraene + 4 NADP+ + 2 allylmercaptan | - |
? | |
ajoene + NADPH + H+ | i.e. (E,Z)-4,5,9-trithiadodeca-1,6,11-triene-9-oxide, substrate and inhibitor | Homo sapiens | 4,5,9-trithiadodeca-1,6,11-triene + NADP+ + H2O | - |
? | |
ajoene + NADPH + H+ | also formation of single-electron reduced product and concomitantly superoxide anion radicals | Homo sapiens | 4,5,9-trithiadodeca-1,6,11-triene + NADP+ + H2O | - |
? | |
diallyl trisulfide + NADPH | - |
Homo sapiens | ? | - |
? | |
GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
diallyl trisulfide | - |
Homo sapiens | |
0.34 | - |
(E)-ajoene | - |
Homo sapiens | |
1.47 | - |
(E)-ajoene | + GSH | Homo sapiens | |
2.6 | - |
(Z)-ajoene | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD enzyme | Homo sapiens | |
NADPH | - |
Homo sapiens |