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Literature summary for 1.8.1.7 extracted from
- Purification of glutathione reductase from chicken liver and investigation of kinetic properties (2005), Appl. Biochem. Biotechnol., 125, 127-138.
General Stability
| General Stability | Organism |
|---|---|
| 1 mM EDTA is required | Gallus gallus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Gallus gallus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
2 * 43000, SDS-PAGE | Gallus gallus |
| 100000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH | Gallus gallus | - |
glutathione + NADP+ | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1714fold from liver homogenate, by ammonium sulfate fractionation, affinity chromatography, and gel filtration | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 120 | - |
purified enzyme from liver | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH | - |
Gallus gallus | glutathione + NADP+ | - |
? | |
| glutathione disulfide + NADPH | optimum ionic strength is 0.75 M using Tris-HCl buffer in presence of EDTA | Gallus gallus | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione reductase | - |
Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Gallus gallus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
optimally stable at | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Gallus gallus | |
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Release 2023.1 (January 2023)
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