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Literature summary for 1.8.1.7 extracted from

  • Bjoerkhem-Bergman, L.; Jonsson, K.; Eriksson, L.C.; Olsson, J.M.; Lehmann, S.; Paul, C.; Bjornstedt, M.
    Drug-resistant human lung cancer cells are more sensitive to selenium cytotoxicity. Effects on thioredoxin reductase and glutathione reductase (2002), Biochem. Pharmacol., 63, 1875-1884.
    View publication on PubMed
Search for more literature for 1.8.1.7

Activating Compound

Activating Compound Comment Organism Structure
doxorubicin differences between drug-sensitive and drug-resistant cells, overview Homo sapiens
Selenite 4fold increase in activity in U-1256 cells, less pronounced in presence of doxorubicin, differences between drug-sensitive and drug-resistant cells, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information cytotoxic effect of selenite on drug-sensitive and drug-resistant cancer cell lines, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH Homo sapiens
-
 glutathione + NADP+
-
 ?
additional information Homo sapiens drug resistance to doxorubicin or selenite does not influence the enzyme activity in lung cancer cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
GLC-4 cell doxorubicin-sensitive lung cancer cell line Homo sapiens
-
GLC-4/ADR cell doxorubicin-insensitive lung cancer cell line expressing the MRP multi-drug resistant protein Homo sapiens
-
HL-60 cell doxorubicin-insensitive leukemic cell line HL-60-R, a subclone of HL-60 Homo sapiens
-
U-1285 cell doxorubicin-sensitive lung cancer cell line Homo sapiens
-
U-1285dox cell doxorubicin-insensitive lung cancer cell line expressing the MRP multi-drug resistant protein Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity in the different cell lines Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH
-
 Homo sapiens glutathione + NADP+
-
 ?
additional information drug resistance to doxorubicin or selenite does not influence the enzyme activity in lung cancer cells Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
glutathione reductase
-
 Homo sapiens
GR
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Homo sapiens