Cloned (Comment) | Organism |
---|---|
in-vitro His-tagged enzyme expression | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cell-free enzyme synthesis, 71% of which is soluble, the enzyme is dimeric, requires FAD as a cofactor, and is more active than the native enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH | Escherichia coli | key enzyme in maintaining the reducing environment in the cell, maintainance of sulfhydryl redox potential in the cytoplasm | glutathione + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from in-vitro expression by nickel chelate affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
139 | - |
purified in vitro-synthesized enzyme, 30°C, 0.002 mM FAD | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
? | |
glutathione disulfide + NADPH | key enzyme in maintaining the reducing environment in the cell, maintainance of sulfhydryl redox potential in the cytoplasm | Escherichia coli | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Escherichia coli |
GR | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | increasing activity between 25°C and 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required for activity | Escherichia coli | |
NADPH | - |
Escherichia coli |