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Literature summary for 1.8.1.7 extracted from
- Purification and characterization of glutathione reductase from rainbow trout (Oncorhynchus mykiss) liver and inhibition effects of metal ions on enzyme activity (2008), Comp. Biochem. Physiol. C, 148, 117-121.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Oncorhynchus mykiss |  |
| Cd2+ | competitive | Oncorhynchus mykiss | |
| Cu2+ | competitive | Oncorhynchus mykiss | |
| Fe3+ | - |
Oncorhynchus mykiss | |
| glutathione | competitive | Oncorhynchus mykiss | |
| Hg2+ | - |
Oncorhynchus mykiss | |
| NADP+ | competitive | Oncorhynchus mykiss | |
| Pb2+ | - |
Oncorhynchus mykiss | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
glutathione disulfide | - |
Oncorhynchus mykiss | |
| 0.055 | - |
NADPH | - |
Oncorhynchus mykiss | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | optimum ionic strength of enzyme is 50 mM TrisHCl | Oncorhynchus mykiss |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
2 * 53000, SDS-PAGE | Oncorhynchus mykiss |
| 105000 | - |
gel filtration | Oncorhynchus mykiss |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oncorhynchus mykiss | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oncorhynchus mykiss |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oncorhynchus mykiss | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.45 | - |
- |
Oncorhynchus mykiss |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH + H+ | - |
Oncorhynchus mykiss | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000, SDS-PAGE | Oncorhynchus mykiss |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Oncorhynchus mykiss |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
enzyme activity in 50 mM Tris-HCl buffer (pH 8.0) is higher than in 50 mM phosphate buffer (pH 8.0) | Oncorhynchus mykiss |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
7 days in 50 mM TrisHCl buffer, 76.25% of initial activity | Oncorhynchus mykiss |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Oncorhynchus mykiss | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.104 | - |
Cd2+ | pH 8.0, 25°C | Oncorhynchus mykiss | |
| 0.117 | - |
Cu2+ | pH 8.0, 25°C | Oncorhynchus mykiss | |
| 10.17 | - |
NADP+ | pH 8.0, 25°C | Oncorhynchus mykiss | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0655 | - |
pH 8.0, 25°C | Oncorhynchus mykiss | Cd2+ | |
| 0.082 | - |
pH 8.0, 25°C | Oncorhynchus mykiss | Cu2+ | |
| 0.122 | - |
pH 8.0, 25°C | Oncorhynchus mykiss | Pb2+ | |
| 0.509 | - |
pH 8.0, 25°C | Oncorhynchus mykiss | Hg2+ | |
| 0.797 | - |
pH 8.0, 25°C | Oncorhynchus mykiss | Fe3+ | |
| 0.804 | - |
pH 8.0, 25°C | Oncorhynchus mykiss | Al3+ | |
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