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Literature summary for 1.8.1.7 extracted from

  • Venancio-Brochi, J.C.; Pereira, L.M.; Calil, F.A.; Teixeira, O.; Baroni, L.; Abreu-Filho, P.G.; Braga, G.U.L.; Nonato, M.C.; Yatsuda, A.P.
    Glutathione reductase A cytoplasmic antioxidant enzyme and a potential target for phenothiazinium dyes in Neospora caninum (2021), Int. J. Biol. Macromol., 187, 964-975 .
    View publication on PubMed
Search for more literature for 1.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Neospora caninum

Inhibitors

Inhibitors Comment Organism Structure
1,9-dimethyl methylene blue
-
 Neospora caninum
methylene blue impairs the activity of recombinant form of the enzyme Neospora caninum
new methylene blue
-
 Neospora caninum
toluidine blue O
-
 Neospora caninum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 NADPH pH 7.5, 37°C Neospora caninum
0.1
-
 glutathione disulfide pH 7.5, 37°C Neospora caninum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Neospora caninum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
 gel filtration Neospora caninum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Neospora caninum
-
 2 glutathione + NADP+
-
 ?
glutathione disulfide + NADPH + H+ Neospora caninum Liverpool
-
 2 glutathione + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Neospora caninum F0VQD6
-
-
Neospora caninum Liverpool F0VQD6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neospora caninum

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Neospora caninum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
 Neospora caninum 2 glutathione + NADP+
-
 ?
glutathione disulfide + NADPH + H+
-
 Neospora caninum Liverpool 2 glutathione + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE, under native conditions the enzyme is found in dimeric form Neospora caninum

Synonyms

Synonyms Comment Organism
glutathione reductase
-
 Neospora caninum
GR
-
 Neospora caninum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62.5
-
 melting temperatute in water Neospora caninum
64.6
-
 melting temperatute in native buffer Neospora caninum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.076
-
 glutathione disulfide pH 7.5, 37°C Neospora caninum
0.08
-
 NADPH pH 7.5, 37°C Neospora caninum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Neospora caninum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0007
-
 pH 7.5, 37°C Neospora caninum new methylene blue
0.0009
-
 pH 7.5, 37°C Neospora caninum toluidine blue O
0.0021
-
 pH 7.5, 37°C Neospora caninum methylene blue
0.011
-
 pH 7.5, 37°C Neospora caninum 1,9-dimethyl methylene blue

General Information

General Information Comment Organism
drug target the enzymed is an important target of phenothiazinium dyes in Neospora caninum proliferation inhibition Neospora caninum
physiological function Neospora caninum causes heavy losses related to abortions in bovine cattle. This parasite develops a complex defense redox system, composed of enzymes as glutathione reductase Neospora caninum