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Literature summary for 1.8.1.9 extracted from
- Purification and characterization of yeast thioredoxin reductase (1973), Biochim. Biophys. Acta, 327, 274-281.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | with NADPH | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0044 | - |
thioredoxin-II | - |
Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Saccharomyces cerevisiae |
| 75000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 103 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thioredoxin + NADP+ | coupled assay with DTNB | Saccharomyces cerevisiae | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | i.e. DTNB | Saccharomyces cerevisiae | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin-II + NADP+ | - |
Saccharomyces cerevisiae | thioredoxin-II disulfide + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 33.3 | - |
NADPH | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2 FAD per dimer | Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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