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Literature summary for 1.8.1.9 extracted from
- Purification of human thioredoxin reductase: properties and characterization by absorption and circular dichroism spectroscopy (1993), Biochemistry, 32, 7271-7277.
General Stability
| General Stability | Organism |
|---|---|
| tightly bound FAD is not replaced during heat treatment or guanidinium hydrochloride | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000, SDS-PAGE | Homo sapiens |
| 130000 | 160000 | sucrose density gradient centrifugation and gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thioredoxin + NADP+ | Homo sapiens | - |
thioredoxin disulfide + NADPH | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | three-dimensional structure | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.8 | - |
purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Homo sapiens | ? | - |
? | |
| thioredoxin + NADP+ | - |
Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | in presence of NADPH, coupled assay | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | i.e. DTNB | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 60000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TR | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
reduction of insulin | Homo sapiens |
| 52 | - |
reduction of DTNB | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 h, no loss of activity | Homo sapiens |
| 65 | - |
above, irreversible loss of activity, no displacement of tightly bound FAD | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| NADPH | - |
Homo sapiens | |
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