Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | 2 pairs of cysteine residues, C32, C35 and C135, C138 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C135S | fluorescence spectroscopic investigation of the interaction with the flavin group | Escherichia coli |
C135S | exchange of 1 cysteine in the active center disulfide, 1 cysteine at position 138 is remaining | Escherichia coli |
C135S/C35S | fluorescence spectroscopic investigation of the interaction with the flavin group | Escherichia coli |
C138S | fluorescence spectroscopic investigation of the interaction with the flavin group | Escherichia coli |
C138S | exchange of 1 cysteine in the active center disulfide, 1 cysteine at position 135 is remaining, very low activity | Escherichia coli |
C138S/C35S | fluorescence spectroscopic investigation of the interaction with the flavin group | Escherichia coli |
C32S | fluorescence spectroscopic investigation of the interaction with the flavin group | Escherichia coli |
C32S | exchange of 1 cysteine in the active center disulfide, 1 cysteine at position 35 is remaining, low activity | Escherichia coli |
C35S | fluorescence spectroscopic investigation of the interaction with the flavin group | Escherichia coli |
C35S | exchange of 1 cysteine in the active center disulfide, 1 cysteine at position 32 is remaining | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | catalytic mechanism | Escherichia coli | |
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | electron transfer in the enzyme complex of apoenzyme, FAD and thioredoxin with NADPH | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |