Cloned (Comment) | Organism |
---|---|
expression of wild-type fusion protein and truncated mutant | Mycobacterium leprae |
subunit complex of enzyme via C135 with thioredoxin C32, mutant complex S135-S32, expression from plasmid | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Escherichia coli |
additional information | thioredoxin is cut off the native fusion protein thioredoxin-thioredoxin reductase resulting in enhanced activity | Mycobacterium leprae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodacetamide | - |
Mycobacterium leprae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mycobacterium leprae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type fusion protein and truncated mutant | Mycobacterium leprae |
wild-type from plasmid | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | oxidation-reduction cycle of thioredoxin | Escherichia coli | |
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | oxidation-reduction cycle of thioredoxin | Mycobacterium leprae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-naphthoquinone + NADPH + H+ | - |
Mycobacterium leprae | ? | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | native thioredoxin-thioredoxin reductase fusion protein | Mycobacterium leprae | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Mycobacterium leprae | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Mycobacterium leprae | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | native thioredoxin-thioredoxin reductase fusion protein | Mycobacterium leprae | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Mycobacterium leprae | thioredoxin disulfide + NADPH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
7 | - |
wild-type fusion protein | Mycobacterium leprae |
9 | - |
around, mutant enzyme, broad optimum | Mycobacterium leprae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | wild-type fusion protein | Mycobacterium leprae |
6.5 | 10 | mutant enzyme, 50% activity at pH 6.5 | Mycobacterium leprae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
FAD | - |
Mycobacterium leprae | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Mycobacterium leprae |