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Literature summary for 1.8.1.9 extracted from

  • Wang, P.F.; Marcinkeviciene, J.; Williams, C.H., Jr.; Blanchard, J.S.
    Thioredoxin Reductase-Thioredoxin Fusion Enzyme from Mycobacterium leprae: Comparison with the Separately Expressed Thioredoxin Reductase (1998), Biochemistry, 37, 16378-16389.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type fusion protein and truncated mutant Mycobacterium leprae
subunit complex of enzyme via C135 with thioredoxin C32, mutant complex S135-S32, expression from plasmid Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information
-
Escherichia coli
additional information thioredoxin is cut off the native fusion protein thioredoxin-thioredoxin reductase resulting in enhanced activity Mycobacterium leprae

Inhibitors

Inhibitors Comment Organism Structure
iodacetamide
-
Mycobacterium leprae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium leprae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type fusion protein and truncated mutant Mycobacterium leprae
wild-type from plasmid Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ oxidation-reduction cycle of thioredoxin Escherichia coli
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ oxidation-reduction cycle of thioredoxin Mycobacterium leprae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-naphthoquinone + NADPH + H+
-
Mycobacterium leprae ?
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH native thioredoxin-thioredoxin reductase fusion protein Mycobacterium leprae 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Escherichia coli 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Mycobacterium leprae 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Escherichia coli 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Mycobacterium leprae 2-nitro-5-thiobenzoate + NADP+
-
?
thioredoxin + NADP+ native thioredoxin-thioredoxin reductase fusion protein Mycobacterium leprae thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay with DTNB Escherichia coli thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay with DTNB Mycobacterium leprae thioredoxin disulfide + NADPH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Escherichia coli
7
-
wild-type fusion protein Mycobacterium leprae
9
-
around, mutant enzyme, broad optimum Mycobacterium leprae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 wild-type fusion protein Mycobacterium leprae
6.5 10 mutant enzyme, 50% activity at pH 6.5 Mycobacterium leprae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Escherichia coli
FAD
-
Mycobacterium leprae
NADPH
-
Escherichia coli
NADPH
-
Mycobacterium leprae