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Literature summary for 1.8.1.9 extracted from

  • Urig, S.; Lieske, J.; Fritz-Wolf, K.; Irmler, A.; Becker, K.
    Truncated mutants of human thioredoxin reductase 1 do not exhibit glutathione reductase activity (2006), FEBS Lett., 580, 3595-3600.
    View publication on PubMed
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
TrxR-16 truncated form of TrxR missing the last 16 C-terminal amino acids, without thioredoxin-reducing activity Homo sapiens
TrxR-16 K29R without thioredoxin-reducing activity Homo sapiens
TrxR-16 K29R/H108Y without thioredoxin-reducing activity Homo sapiens
TrxR-16 K29R/H108Y/A119N/V478E without thioredoxin-reducing activity Homo sapiens
U498C 1.4fold higher GSSG-reducing activity compared to the TrxR-16 enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
 NADPH wild type enzyme Homo sapiens
0.088
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme Homo sapiens
0.7
-
 NADPH mutant U498C Homo sapiens
0.7
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant U498C Homo sapiens
1.1
-
 NADPH TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
1.1
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
2.4
-
 NADPH TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
2.4
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
3.3
-
 NADPH TrxR-16 mutant K29R Homo sapiens
3.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R Homo sapiens
4.5
-
 NADPH TrxR-16 mutant K29R/H108Y Homo sapiens
4.5
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
 TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
0.007
-
 TrxR-16 mutant K29R/H108Y Homo sapiens
0.016
-
 TrxR-16 mutant K29R Homo sapiens
0.025
-
 TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
0.035
-
 mutant U498C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?
GSSG + NADPH
-
 Homo sapiens GSH + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
thioredoxin reductase 1
-
 Homo sapiens
TrxR1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 5,5'-dithiobis(2-nitrobenzoic acid) mutant U498C Homo sapiens
0.018
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y/A119N/V478E Homo sapiens
0.075
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R/H108Y Homo sapiens
0.52
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16 mutant K29R Homo sapiens
0.55
-
 5,5'-dithiobis(2-nitrobenzoic acid) TrxR-16, TrxR lacking the last 16 C-terminal amino acids Homo sapiens
33.33
-
 5,5'-dithiobis(2-nitrobenzoic acid) wild type enzyme Homo sapiens