Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-chloro-2,4-dinitrobenzene | 0.1 mM | Mus musculus | |
diphenylene iodonium | IC50: 0.001 mM | Mus musculus | |
sodium aurothiomalate | 0.1 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effects of paraquat on reaction kinetics of NADH and NADPH in lung cell extracts | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the enzyme can promote oxidative stress by redox cycling of paraquat: paraquat + O2 + NADPH + H+ --> paraquat radical + O2- radical + NADP+ | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
2',5'-ADP-agarose affinity chromatography and Superose size exclusion chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung epithelium | - |
Mus musculus | - |
lung epithelium | MLE-15 cell line | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
additional information | the enzyme can promote oxidative stress by redox cycling of paraquat: paraquat + O2 + NADPH + H+ --> paraquat radical + O2- radical + NADP+ | Mus musculus | ? | - |
? | |
paraquat + NAD(P)H | - |
Mus musculus | paraquat radical + NAD(P)+ | - |
r | |
paraquat radical + O2 | - |
Mus musculus | paraquat + O2- | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H:paraquat oxidoreductase | - |
Mus musculus |
thioredoxin reductase | - |
Mus musculus |
TrxR | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mus musculus | |
NADH | - |
Mus musculus | |
NADPH | - |
Mus musculus | |
NADPH | approximately four times more activity was evident with NADPH when compared to NADH | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
IC50: 0.001 mM | Mus musculus | diphenylene iodonium |