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Literature summary for 1.8.1.9 extracted from

  • Eckenroth, B.E.; Rould, M.A.; Hondal, R.J.; Everse, S.J.
    Structural and biochemical studies reveal differences in the catalytic mechanisms of mammalian and Drosophila melanogaster thioredoxin reductases (2007), Biochemistry, 46, 4694-4705.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mus musculus
expression in Escherichia coli Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop diffusion method at 20°C, X-ray crystal structure of DmTR at 2.4 A resolution in which the enzyme was truncated to remove the C-terminal tripeptide sequence Cys-Cys-Ser Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information the truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid shows no activity with thioredoxin disulfide + NADPH Mus musculus
additional information truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid shows no activity with thioredoxin disulfide + NADPH Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 thioredoxin disulfide full-length mouse enzyme with C-terminal sequence SCCS Mus musculus
0.0676
-
 thioredoxin disulfide full-length mouse enzyme with C-terminal sequence GCUG Mus musculus
0.123
-
 thioredoxin disulfide full-length mouse enzyme with C-terminal sequence GCCG Mus musculus
0.141
-
 thioredoxin disulfide full-length Drosophila enzyme with C-terminal sequence SCCS Drosophila melanogaster
0.17
-
 5,5'-dithiobis(2-nitrobenzoic acid) full-length Drosophila enzyme with C-terminal sequence SCCS Drosophila melanogaster
0.47
-
 5,5'-dithiobis(2-nitrobenzoic acid) full-length mouse enzyme with C-terminal sequence GCUG Mus musculus
0.92
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid Drosophila melanogaster
2.72
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information DmTR shows high catalytic activity without the presence of a selenocysteine Drosophila melanogaster
additional information DmTR shows high catalytic activity without the presence of a selenocysteine Mus musculus

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster P91938
-
-
Mus musculus Q8BTW3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Drosophila melanogaster
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Drosophila melanogaster 2-nitro-5-thiobenzoate + NADP+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Mus musculus 2-nitro-5-thiobenzoate + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Drosophila melanogaster thioredoxin + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Mus musculus thioredoxin + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
DmTR
-
 Drosophila melanogaster
mTR3
-
 Mus musculus
thioredoxin reductase
-
 Drosophila melanogaster
thioredoxin reductase
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.083
-
 thioredoxin disulfide full-length mouse enzyme with C-terminal sequence SCCS Mus musculus
0.13
-
 thioredoxin disulfide full-length mouse enzyme with C-terminal sequence GCCG Mus musculus
1.6
-
 5,5'-dithiobis(2-nitrobenzoic acid) full-length Drosophila enzyme with C-terminal sequence SCCS Drosophila melanogaster
2.4
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated enzyme (missing residues CCS from the C-terminus) so that Ser488 is the C-terminal amino acid Drosophila melanogaster
5.8
-
 thioredoxin disulfide full-length Drosophila enzyme with C-terminal sequence SCCS Drosophila melanogaster
15.6
-
 5,5'-dithiobis(2-nitrobenzoic acid) truncated mutant enzyme (missing residues CUG from the C-terminus) so that Gly521 is the C-terminal amino acid Mus musculus
20.83
-
 5,5'-dithiobis(2-nitrobenzoic acid) full-length mouse enzyme with C-terminal sequence GCUG Mus musculus
37
-
 thioredoxin disulfide full-length mouse enzyme with C-terminal sequence GCUG Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Drosophila melanogaster
NADPH
-
 Mus musculus