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Literature summary for 1.8.1.9 extracted from

  • Machida, T.; Kato, E.; Ishibashi, A.; Sato, J.; Kawasaki, S.; Niimura, Y.; Honjoh, K.; Miyamoto, T.
    Expression pattern of a chloroplast NADPH-dependent thioredoxin reductase in Chlorella vulgaris during hardening and its interaction with 2-Cys peroxiredoxin (2009), Biosci. Biotechnol. Biochem., 73, 695-701.
    View publication on PubMed
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Chlorella vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlorella vulgaris 9507
-

Organism

Organism UniProt Comment Textmining
Chlorella vulgaris B9ZYY5
-
-
Chlorella vulgaris Beijerinck IAM C-27 B9ZYY5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Chlorella vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + dithiothreitol
-
 Chlorella vulgaris 2-nitro-5-thiobenzoate + oxidized dithiothreitol
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + dithiothreitol
-
 Chlorella vulgaris Beijerinck IAM C-27 2-nitro-5-thiobenzoate + oxidized dithiothreitol
-
 ?
additional information NTRC shows both NADPH-dependent thioredoxin reductase and thioredoxin-like dithiol-disulfide oxidoreductase activities Chlorella vulgaris ?
-
 ?
additional information NTRC shows both NADPH-dependent thioredoxin reductase and thioredoxin-like dithiol-disulfide oxidoreductase activities Chlorella vulgaris Beijerinck IAM C-27 ?
-
 ?

Synonyms

Synonyms Comment Organism
NTR
-
 Chlorella vulgaris
NTRC
-
 Chlorella vulgaris
thioredoxin reductase
-
 Chlorella vulgaris

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Chlorella vulgaris
NADPH dependent on Chlorella vulgaris

Expression

Organism Comment Expression
Chlorella vulgaris the transcript of NTRC increases throughout 24 h hardening, whereas the encoded protein amount and total NTR activity decrease once and the increase during hardening up

General Information

General Information Comment Organism
physiological function NTRC is maintained at a constant level during hardening and functions as an antioxidant with 2-Cys peroxiredoxin in the acquisition of freezing tolerance of Chlorella Chlorella vulgaris