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Literature summary for 1.8.1.9 extracted from

  • Wulff, R.P.; Lundqvist, J.; Rutsdottir, G.; Hansson, A.; Stenbaek, A.; Elmlund, D.; Elmlund, H.; Jensen, P.E.; Hansson, M.
    The activity of barley NADPH-dependent thioredoxin reductase C is independent of the oligomeric state of the protein: tetrameric structure determined by cryo-electron microscopy (2011), Biochemistry, 50, 3713-3723.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 pLysS cells Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
etioplast
-
Hordeum vulgare 9513
-
soluble
-
Hordeum vulgare
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54662
-
4 * 54662, calculated from sequence of cDNA Hordeum vulgare
63000
-
4 * 63000, SDS-PAGE Hordeum vulgare
200000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thioredoxin disulfide + NADPH + H+ Hordeum vulgare
-
thioredoxin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare B0FXK2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography and Superose 6 gel filtration Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoicacid) + NADPH + H+
-
Hordeum vulgare 5'-thionitrobenzoic acid + NADP+
-
?
thioredoxin disulfide + NADPH + H+
-
Hordeum vulgare thioredoxin + NADP+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 63000, SDS-PAGE Hordeum vulgare
homotetramer 4 * 54662, calculated from sequence of cDNA Hordeum vulgare

Synonyms

Synonyms Comment Organism
NADPH-dependent thioredoxin reductase C
-
Hordeum vulgare
NTRC
-
Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
FAD
-
Hordeum vulgare
NADPH
-
Hordeum vulgare