Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 pLysS cells | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
etioplast | - |
Hordeum vulgare | 9513 | - |
soluble | - |
Hordeum vulgare | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54662 | - |
4 * 54662, calculated from sequence of cDNA | Hordeum vulgare |
63000 | - |
4 * 63000, SDS-PAGE | Hordeum vulgare |
200000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin disulfide + NADPH + H+ | Hordeum vulgare | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | B0FXK2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and Superose 6 gel filtration | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoicacid) + NADPH + H+ | - |
Hordeum vulgare | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Hordeum vulgare | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 63000, SDS-PAGE | Hordeum vulgare |
homotetramer | 4 * 54662, calculated from sequence of cDNA | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent thioredoxin reductase C | - |
Hordeum vulgare |
NTRC | - |
Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Hordeum vulgare | |
NADPH | - |
Hordeum vulgare |