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Literature summary for 1.8.1.9 extracted from

  • Arias, D.G.; Regner, E.L.; Iglesias, A.A.; Guerrero, S.A.
    Entamoeba histolytica thioredoxin reductase: molecular and functional characterization of its atypical properties (2012), Biochim. Biophys. Acta, 1820, 1859-1866.
    View publication on PubMed
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Entamoeba histolytica
NAD+ NAD+ acts as poor competitive inhibitor respect to both NADPH and NADH Entamoeba histolytica
NADP+ better competitive inhibitor than NAD+ Entamoeba histolytica
Zn2+
-
 Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.0023
-
 thioredoxin 41 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.0028
-
 thioredoxin 8 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.0029
-
 thioredoxin 8 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.0036
-
 thioredoxin 41 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.0047
-
 thioredoxin with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.0062
-
 thioredoxin with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.023
-
 NADH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.1
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.4
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64000, SDS-PAGE Entamoeba histolytica
78000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Entamoeba histolytica in addition, the enzyme exhibits NAD(P)H dependent oxidase activity, which generates hydrogen peroxide from molecular oxygen ?
-
 ?
thioredoxin + NADP+ Entamoeba histolytica
-
 thioredoxin disulfide + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Entamoeba histolytica 5'-thionitrobenzoic acid + NAD+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Entamoeba histolytica 5'-thionitrobenzoic acid + NADP+
-
 ?
additional information in addition, the enzyme exhibits NAD(P)H dependent oxidase activity, which generates hydrogen peroxide from molecular oxygen Entamoeba histolytica ?
-
 ?
thioredoxin + NADP+
-
 Entamoeba histolytica thioredoxin disulfide + NADPH + H+
-
 ?
thioredoxin 41 + NADP+
-
 Entamoeba histolytica thioredoxin 41 disulfide + NADPH + H+
-
 ?
thioredoxin 8 + NADP+
-
 Entamoeba histolytica thioredoxin 8 disulfide + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 64000, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
TrxR
-
 Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 NADH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.23
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.25
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.25
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.5
-
 thioredoxin with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.25
-
 thioredoxin with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
2
-
 thioredoxin 41 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
2.2
-
 thioredoxin 41 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
2.6
-
 thioredoxin 8 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
2.7
-
 thioredoxin 8 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol FAD is bound per mol subunit Entamoeba histolytica
NADH
-
 Entamoeba histolytica
NADPH higher affinity and catalytic efficiency with NADPH compared to NADH Entamoeba histolytica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 NADP+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.026
-
 NADP+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.313
-
 NAD+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.478
-
 NAD+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0025
-
 in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica Cu2+
0.0034
-
 in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica Zn2+

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.17
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
0.23
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
75
-
 thioredoxin with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
84
-
 NADH with 5,5'-dithiobis(2-nitrobenzoicacid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
140
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
260
-
 thioredoxin with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
600
-
 thioredoxin 41 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
880
-
 thioredoxin 41 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
890
-
 thioredoxin 8 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
950
-
 thioredoxin 8 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica