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Literature summary for 1.8.1.9 extracted from
- Functional thioredoxin reductase from pathogenic and free-living Leptospira spp. (2016), Free Radic. Biol. Med., 97, 1-13 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
thioredoxin disulfide | pH 7.0, 30°C | Leptospira interrogans serovar Copenhageni |  |
| 0.0022 | - |
NADPH | pH 7.0, 30°C | Leptospira interrogans serovar Copenhageni | |
| 0.0067 | - |
thioredoxin disulfide | pH 7.0, 30°C | Leptospira biflexa serovar Patoc | |
| 0.007 | - |
NADPH | pH 7.0, 30°C | Leptospira biflexa serovar Patoc | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Leptospira interrogans serovar Copenhageni |
| 70000 | - |
gel filtration | Leptospira biflexa serovar Patoc |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leptospira biflexa serovar Patoc | B0SKV7 | - |
- |
| Leptospira biflexa serovar Patoc ATCC 23582 | B0SKV7 | - |
- |
| Leptospira interrogans serovar Copenhageni | Q72SB4 | - |
- |
| Leptospira interrogans serovar Copenhageni Fiocruz L1-130 | Q72SB4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
| 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Leptospira biflexa serovar Patoc | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
| 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni Fiocruz L1-130 | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
| 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+ | - |
Leptospira biflexa serovar Patoc ATCC 23582 | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
| bis-gamma-glutamyl cystine + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni | gamma-glutamyl cystine + NADP+ | - |
? | |
| bis-gamma-glutamyl cystine + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni Fiocruz L1-130 | gamma-glutamyl cystine + NADP+ | - |
? | |
| cystine + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni | 2 cysteine + NADP+ | - |
? | |
| glutathione disulfide + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni | glutathione + NADP+ | - |
? | |
| glutathione disulfide + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni Fiocruz L1-130 | glutathione + NADP+ | - |
? | |
| lipoamide disulfide + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni | lipoamide + NADP+ | - |
? | |
| additional information | no substrates: ferredoxin, flavodoxin | Leptospira interrogans serovar Copenhageni | ? | - |
? | |
| additional information | no substrates: ferredoxin, flavodoxin | Leptospira biflexa serovar Patoc | ? | - |
? | |
| additional information | no substrates: ferredoxin, flavodoxin | Leptospira interrogans serovar Copenhageni Fiocruz L1-130 | ? | - |
? | |
| additional information | no substrates: ferredoxin, flavodoxin | Leptospira biflexa serovar Patoc ATCC 23582 | ? | - |
? | |
| peroxiredoxin + NADPH + H+ | - |
Leptospira interrogans serovar Copenhageni | peroxiredoxin disulfide + NADP+ | - |
? | |
| thioredoxin disulfide + NADPH | - |
Leptospira interrogans serovar Copenhageni | thioredoxin + NADP+ | - |
? | |
| thioredoxin disulfide + NADPH | - |
Leptospira biflexa serovar Patoc | thioredoxin + NADP+ | - |
? | |
| thioredoxin disulfide + NADPH | - |
Leptospira interrogans serovar Copenhageni Fiocruz L1-130 | thioredoxin + NADP+ | - |
? | |
| thioredoxin disulfide + NADPH | - |
Leptospira biflexa serovar Patoc ATCC 23582 | thioredoxin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34000, calculated | Leptospira interrogans serovar Copenhageni |
| dimer | 2 * 34000, calculated | Leptospira biflexa serovar Patoc |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrxB | - |
Leptospira interrogans serovar Copenhageni |
| TrxB | - |
Leptospira biflexa serovar Patoc |
| TrxR1 | - |
Leptospira biflexa serovar Patoc |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.01 | - |
NADPH | pH 7.0, 30°C | Leptospira biflexa serovar Patoc | |
| 6 | - |
NADPH | pH 7.0, 30°C | Leptospira interrogans serovar Copenhageni | |
| 7.7 | - |
thioredoxin disulfide | pH 7.0, 30°C | Leptospira interrogans serovar Copenhageni | |
| 13.3 | - |
thioredoxin disulfide | pH 7.0, 30°C | Leptospira biflexa serovar Patoc | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Leptospira interrogans serovar Copenhageni |
| 7 | - |
- |
Leptospira biflexa serovar Patoc |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Leptospira interrogans serovar Copenhageni | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 590 | - |
NADPH | pH 7.0, 30°C | Leptospira biflexa serovar Patoc | |
| 1900 | - |
thioredoxin disulfide | pH 7.0, 30°C | Leptospira biflexa serovar Patoc | |
| 2800 | - |
NADPH | pH 7.0, 30°C | Leptospira interrogans serovar Copenhageni | |
| 3700 | - |
thioredoxin disulfide | pH 7.0, 30°C | Leptospira interrogans serovar Copenhageni | |
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