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Literature summary for 1.8.1.9 extracted from
- A novel twist on molecular interactions between thioredoxin and nicotinamide adenine dinucleotide phosphate-dependent thioredoxin reductase (2014), Proteins, 82, 607-619 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
- |
Hordeum vulgare subsp. vulgare |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modelling of the complex with thioredoxin | Hordeum vulgare subsp. vulgare |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C145S | active site cysteine residue. Kinetic data | Hordeum vulgare subsp. vulgare |
| C148S | residue Cys148 probably performs an initial nucleophilic attack on the active site disulfide in thioredoxin disulfide. Kinetic data | Hordeum vulgare subsp. vulgare |
| Delta42-47 | FAD-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| G222D/A223G/G224E | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| G225R/G226D | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| G225R/G226D/P227V | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| M43A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin. Kinetic data | Hordeum vulgare subsp. vulgare |
| N139A | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| N45A/D46A | FAD-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| R140A | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
| W42A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin. Kinetic data | Hordeum vulgare subsp. vulgare |
| W42A/M43A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin | Hordeum vulgare subsp. vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00041 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00048 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.00052 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00057 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00067 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00068 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00073 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0008 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00105 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00117 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00123 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00128 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0014 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00146 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0016 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0022 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0024 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.00343 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0042 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0141 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0155 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.016 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0305 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0457 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.0543 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 0.06 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.149 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q39243 | - |
- |
| Hordeum vulgare subsp. vulgare | A9LN30 | isoform Ntr2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Arabidopsis thaliana thioredoxin 3 + NADP+ | - |
Arabidopsis thaliana | Arabidopsis thaliana thioredoxin 3 disulfide + NADPH + H+ | - |
? |  |
| Arabidopsis thaliana thioredoxin 3 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Arabidopsis thaliana thioredoxin 3 disulfide + NADPH + H+ | - |
? | |
| Escherichia coli thioredoxin + NADP+ | - |
Arabidopsis thaliana | Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
? | |
| Escherichia coli thioredoxin + NADP+ | - |
Hordeum vulgare subsp. vulgare | Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 1 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 mutant E86A + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant E86A disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 mutant G47P + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 mutant G47P disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 mutant G57P + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant G57P disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 mutant I51G + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 mutant I51G disulfide + NADPH + H+ | - |
? | |
| Hordeum vulgare thioredoxin 2 mutant I51G + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant I51G disulfide + NADPH + H+ | - |
? | |
| additional information | binding of NADP+ enhances the ability of Ntr2 to bind thioredoxin | Hordeum vulgare subsp. vulgare | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTR2 | - |
Hordeum vulgare subsp. vulgare |
| NtrB | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 2.6 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 3.9 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 4.2 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 4.2 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 4.8 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5.1 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5.3 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5.9 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 7.3 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 7.9 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 7.9 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 8 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 8.1 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 8.5 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 9.4 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 10.4 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 10.4 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 10.7 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 11 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 11.4 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 12.1 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 14.8 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 15.3 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 15.9 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Arabidopsis thaliana | |
| FAD | - |
Hordeum vulgare subsp. vulgare | |
| NADP+ | - |
Arabidopsis thaliana | |
| NADP+ | - |
Hordeum vulgare subsp. vulgare | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 63.1 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 140 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
| 228 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 282 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 361 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 713 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1030 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1050 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1490 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1630 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 1710 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 2550 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 3300 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 4240 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5340 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5410 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 5490 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 6000 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 6180 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 6330 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 6800 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 7460 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 9230 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 9620 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 9840 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 10200 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
| 12300 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |
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