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Literature summary for 1.8.1.B1 extracted from

  • Wu, Z.; Hou, Y.; Dai, Z.; Hu, C.A.; Wu, G.
    Metabolism, nutrition, and redox signaling of hydroxyproline (2017), Antioxid. Redox Signal., 30, 674-682.
    View publication on PubMed
Search for more literature for 1.8.1.B1

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.7 A resolution, space group of P43212, one physiological homodimer per asymmetric unit. Structure displays distinct binding sites for thioredoxin and the glutaredoxin domain, a single glutathione disulfide reduction site in the Grx domain, and rotation of the glutaredoxin domain toward the Sec-containing redox active site Echinococcus granulosus

Protein Variants

Protein Variants Comment Organism
C519S about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid) Echinococcus granulosus
C574S about 30% of wild-type activity with both substrates glutathione and 5,5'-dithio-bis(2-nitrobenzoic acid) Echinococcus granulosus

Inhibitors

Inhibitors Comment Organism Structure
[mu-[1-(hydroxy-1kappaO)-2-(sulfanyl-1kappaS)pyridin-1-iumato(2-)]]-[mu-[1-(hydroxy-2kappaO)-2-(sulfanyl-1kappaS)pyridin-1-iumato(2-)]]bis(triphenylphosphane)digold a single gold atom binds to Cys519 and Cys573 in the AuI-enzyme complex. 75–80% inhibition of thioredoxin and glutathione reductase activities is achieved at a 1:1 concentration ratio of inhibitor:enzyme Echinococcus granulosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 glutathione disulfide mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.013
-
 glutathione disulfide mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.015
-
 glutathione disulfide wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.033
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.034
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.038
-
 5,5'-dithio-bis(2-nitrobenzoic acid) wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus

Organism

Organism UniProt Comment Textmining
Echinococcus granulosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Echinococcus granulosus 5'-thionitrobenzoic acid + NADP+
-
 ?
glutathione disulfide + NADPH + H+
-
 Echinococcus granulosus glutathione + NADP+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 glutathione disulfide wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus
5
-
 glutathione disulfide mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
5.6
-
 glutathione disulfide mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
10.2
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
12
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
55
-
 5,5'-dithio-bis(2-nitrobenzoic acid) wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Echinococcus granulosus
NADPH
-
 Echinococcus granulosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.31
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.35
-
 5,5'-dithio-bis(2-nitrobenzoic acid) mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.38
-
 glutathione disulfide mutant C574S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
0.47
-
 glutathione disulfide mutant C519S, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.45
-
 5,5'-dithio-bis(2-nitrobenzoic acid) wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus
1.87
-
 glutathione disulfide wild-type, pH 7.0, temperature not specified in the publication Echinococcus granulosus