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Literature summary for 1.8.1.B1 extracted from
- Purification and kinetic analysis of cytosolic and mitochondrial thioredoxin glutathione reductase extracted from Taenia solium cysticerci (2015), Exp. Parasitol., 149, 65-73 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| auranofin | both cytolsolic and mitochondrial isoform | Taenia solium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
NADPH | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.004 | - |
glutathione disulfide | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.0063 | - |
glutathione disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.045 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.048 | - |
NADPH | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.067 | - |
thioredoxin disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.088 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.184 | - |
glutathione-hydroxyethyl disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.41 | - |
glutathione-hydroxyethyl disulfide | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | isoform cTGR | Taenia solium | 5829 | - |
| mitochondrion | isoform mTGR | Taenia solium | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
gel filtration, both cytosolic and mitochondrial isoform | Taenia solium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Taenia solium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Taenia solium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 980 | - |
cytosolic isoform, substrate glutathione disulfide, pH 7.8, temperature not specified in the publication | Taenia solium |
| 1300 | - |
mitochondrial isoform, substrate glutathione disulfide, pH 7.8, temperature not specified in the publication | Taenia solium |
| 2140 | - |
cytosolic isoform, substrate 5,5'-dithiobis(2-nitrobenzoic acid), pH 7.8, temperature not specified in the publication | Taenia solium |
| 10100 | - |
mitochondrial isoform, substrate 5,5'-dithiobis(2-nitrobenzoic acid), pH 7.8, temperature not specified in the publication | Taenia solium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Taenia solium | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| glutathione disulfide + NADPH + H+ | - |
Taenia solium | 2 glutathione + NADP+ | - |
? | |
| glutathione-hydroxyethyl disulfide + NADPH + H+ | - |
Taenia solium | glutathione + 2-sulfanylethan-1-ol + NADP+ | - |
? | |
| insulin disulfide + NADPH + H+ | - |
Taenia solium | insulin + NADP+ | - |
? | |
| thioredoxin disulfide + NADPH + H+ | Escherichia coli thioredoxin disulfide | Taenia solium | thioredoxin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 62000, SDS-PAGE, both cytosolic and mitochondrial isoform | Taenia solium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
both cytolsolic and mitochondrial isoform | Taenia solium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
both cytolsolic and mitochondrial isoform, complete loss of activity | Taenia solium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
thioredoxin disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 0.96 | - |
glutathione disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 1.62 | - |
glutathione disulfide | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 1.64 | - |
NADPH | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 1.85 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 5.13 | - |
glutathione-hydroxyethyl disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 12.6 | - |
NADPH | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 12.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 163.5 | - |
glutathione-hydroxyethyl disulfide | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
cytosolic isoform | Taenia solium |
| 8.5 | - |
mitochondrial isoform, substrate 5,5'-dithiobis(2-nitrobenzoic acid) | Taenia solium |
| 9.5 | - |
mitochondrial isoform, substrate glutathione disulfide | Taenia solium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Taenia solium | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.2 | - |
thioredoxin disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 21 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 28 | - |
glutathione-hydroxyethyl disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 34 | - |
NADPH | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 150 | - |
glutathione disulfide | cytosolic isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 390 | - |
glutathione-hydroxyethyl disulfide | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 420 | - |
glutathione disulfide | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 2800 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
| 7900 | - |
NADPH | mitochondrial isoform, pH 7.8, temperature not specified in the publication | Taenia solium | |
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