Literature summary for 1.8.1.B1 extracted from

Gupta, A.; Kesherwani, M.; Velmurugan, D.; Tripathi, T.
Fasciola gigantica thioredoxin glutathione reductase Biochemical properties and structural modeling (2016), Int. J. Biol. Macromol., 89, 152-160 .

Search for more literature for 1.8.1.B1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Fasciola gigantica

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeled structure is a functional dimer, and contains a Grx domain (residues 2183), a FAD/NADPH-binding domain or TrxR domain (residues 109450) and a dimerization domain (residues 470598)	Fasciola gigantica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00434	-	5,5'-dithiobis(2-nitrobenzoic acid)	pH 7.5, 25°C	Fasciola gigantica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
130000	-	gel filtration	Fasciola gigantica

Organism

Organism	UniProt	Comment	Textmining
Fasciola gigantica	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+	-	Fasciola gigantica	2-nitro-5-thiobenzoate + NADP+	-	?
glutathione disulfide + NADPH + H+	enzyme displays hysteretic behavior	Fasciola gigantica	2 glutathione + NADP+	-	?
oxidized insulin + NADPH + H+	-	Fasciola gigantica	reduced insulin + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 66000, calculated and SDS-PAGE	Fasciola gigantica

Cofactor

Cofactor	Comment	Organism	Structure
FAD	the flavin ring of FAD cofactor binds in a hydrophobic cleft formed by Cys154, Val157, Gly158, Cys159, Pro572, Phe474, and Pro443	Fasciola gigantica
NADPH	nicotinamide moiety binds up in the hydrophobic cleft formed by Val348, Ile266, Pro264,Val292, Met315, Val316, and Pro349, etc. and the diphosphate forms H-bond with Ile391, Ser318, and Arg317. The ribose sugar formes H-bonds with Arg262, Arg322, and Tyr296	Fasciola gigantica

pI Value

Organism	Comment	pI Value Maximum	pI Value
Fasciola gigantica	calculated	-	6.3

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Release 2023.1 (January 2023)