Cloned (Comment) | Organism |
---|---|
the human enzyme is recombinantly produced (hTGR) by fusing its open reading frame with a bacterial selenocysteine insertion sequence element and coexpressing the construct in Escherichia coli together with the selA, selB, and selC genes. Additionally, the Sec->Cys mutant (hTGRU642C) of the full-length protein, the isolated TrxR domain (hTGR151-643) and the Grx domain containing a monothiol active site (hTGR1-150) are produced | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
U642C | in the wild-type enzyme, the KM value for NADPH is significantly higher than in the mutant enzyme, whereas the KM value for the oxidizing substrate thioredoxin disulfide in the wild-type enzyme is significantly lower than the Km-value of the mutant enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
NADPH | mutant enzyme U642C, cosubstrate: thioredoxin disulfide, pH 7.4, 25°C | Homo sapiens | |
0.0075 | - |
thioredoxin disulfide | recombinant wild-type enzyme, pH 7.4, 25°C | Homo sapiens | |
0.0166 | - |
NADPH | recombinant wild-type enzyme, cosubstrate: thioredoxin disulfide, pH 7.4, 25°C | Homo sapiens | |
0.0205 | - |
thioredoxin disulfide | mutant enzyme U642C, pH 7.4, 25°C | Homo sapiens | |
0.0208 | - |
NADPH | recombinant wild-type enzyme, cosubstrate: glutathione disulfide, pH 6.9, 25°C | Homo sapiens | |
0.0208 | - |
glutathione disulfide | recombinant wild-type enzyme, pH 6.9, 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | selenoprotein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | Homo sapiens | - |
2 glutathione + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Homo sapiens | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q86VQ6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinantly produced human enzyme (hTGR), the Sec->Cys mutant (hTGRU642C) of the full-length protein, the isolated TrxR domain (hTGR151-643) and the Grx domain containing a monothiol active site (hTGR1-150) are produced | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0125 | - |
substrate: glutathione disulfide, recombinant wild-type enzyme, pH 6.9, 25°C | Homo sapiens |
0.0599 | - |
substrate: thioredoxin disulfide, mutant enzyme U642C,pH 7.4, 25°C | Homo sapiens |
0.0947 | - |
substrate: thioredoxin disulfide, recombinant wild-type enzyme, pH 7.4, 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Homo sapiens | 2 glutathione + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Homo sapiens | thioredoxin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TGR | - |
Homo sapiens |
thioredoxin glutathione reductase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADPH | 25°C, pH 7.4, mutant enzyme U642C, cosubstrate: thioredoxin disulfide | Homo sapiens | |
0.02 | - |
thioredoxin disulfide | 25°C, pH 7.4, mutant enzyme U642C | Homo sapiens | |
0.11 | - |
glutathione disulfide | 25°C, pH 6.9, recombinant wild-type enzyme | Homo sapiens | |
0.14 | - |
NADPH | 25°C, pH 6.9, recombinant wild-type enzyme, cosubstrate: thioredoxin disulfide | Homo sapiens | |
0.18 | - |
thioredoxin disulfide | 25°C, pH 7.4, recombinant wild-type enzyme | Homo sapiens | |
0.2 | - |
NADPH | 25°C, pH 7.4, recombinant wild-type enzyme, cosubstrate: thioredoxin disulfide | Homo sapiens |