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Literature summary for 1.8.2.1 extracted from
- Catalytic voltammetry of the molybdoenzyme sulfite dehydrogenase from Sinorhizobium meliloti (2014), J. Phys. Chem. B, 118, 7091-7099 .
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Sinorhizobium meliloti | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mo4+ | molybdenum enzyme. Sulfite oxidizing enzymes (regardless of origin) share a common active site comprising a dioxido-MoVI moiety chelated by a molybdopterin dithiolene ligand in addition to a cysteinyl S-donor. The equatorial oxido ligand is the one transferred to sulfite during its 2-electron O-atom transfer reaction | Sinorhizobium meliloti |  |
| additional information | the enzyme bears no heme cofactor | Sinorhizobium meliloti |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
- |
Sinorhizobium meliloti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + 2 ferricytochrome c + H2O | Sinorhizobium meliloti | - |
sulfate + 2 ferrocytochrome c + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| sulfite + 2 ferricytochrome c + H2O = sulfate + 2 ferrocytochrome c + 2 H+ | mechanism of electrochemically mediated, enzyme SorT-catalyzed sulfite oxidation | Sinorhizobium meliloti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
pH 8.0, 22°C, with horse heart cytochrome c | Sinorhizobium meliloti |
| 33.4 | - |
pH 8.0, 22°C, with SorU | Sinorhizobium meliloti |
| 470 | - |
pH 8.0, 22°C, with ferricyanide | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | favorable protein-protein interactions between SorT and c-type cytochrome SorU, i.e. Smc04048, lead to productive electron transfer and catalytic activity. No activity with ferrous horse heart cytochrome c, and no activity with O2 as an sulfite oxidase | Sinorhizobium meliloti | ? | - |
? | |
| sulfite + 2 ferricytochrome c + H2O | - |
Sinorhizobium meliloti | sulfate + 2 ferrocytochrome c + 2 H+ | - |
? | |
| sulfite + 2 ferrocene methanol + H2O | - |
Sinorhizobium meliloti | sulfate + 2 reduced ferrocene methanol + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Sinorhizobium meliloti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SorT | - |
Sinorhizobium meliloti |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Sinorhizobium meliloti |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
20 min, inactivation | Sinorhizobium meliloti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the lack of a plateau prevents the determination of a genuine pH optimum, an apparently linear decrease in activity as a function of pH is not interpretable by standard models of pH-dependent enzyme activity which predict sigmoidal or bell-shaped profiles | Sinorhizobium meliloti |
| 8 | - |
assay at | Sinorhizobium meliloti |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the greatest loss in activity occurs while the enzyme is at pH 10, while in the range above pH 6 and below pH 9 there is no significant variation, inactivation at pH 11.0 | Sinorhizobium meliloti |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
irreversible time-dependent loss of catalytic activity | Sinorhizobium meliloti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | c-type cytochrome SorU, i.e. Smc04048 | Sinorhizobium meliloti | |
| additional information | ferrocene methanol (in its oxidized ferrocenium form) is utilized as an artificial electron acceptor for the catalytic SorT sulfite oxidation reaction | Sinorhizobium meliloti |
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