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Literature summary for 1.8.2.2 extracted from
- Purification and properties of thiosulfate reductase from Desulfovibrio vulgaris, Miyazaki F (1985), J. Biochem., 97, 1025-1032.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | 0.1 mM, 148% of initial activity | Desulfovibrio vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-bipyridine | 1 mM, 22% residual activity | Desulfovibrio vulgaris | |
| AsO2- | 1 mM, 50% residual activity | Desulfovibrio vulgaris | |
| EDTA | 1 mM, 52% residual activity | Desulfovibrio vulgaris | |
| iodoacetate | 1 mM, 51% residual activity | Desulfovibrio vulgaris | |
| o-phenanthroline | 1 mM, 12% residual activity | Desulfovibrio vulgaris | |
| Sodium azide | 1 mM, 74% residual activity | Desulfovibrio vulgaris | |
| thiosulfate | inhibitory above 0.75 mM | Desulfovibrio vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 0.1 mM, 142% of initial activity | Desulfovibrio vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
1 * 80000, SDS-PAGE | Desulfovibrio vulgaris |
| 85000 | 89000 | gel filtration | Desulfovibrio vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio vulgaris | - |
- |
- |
| Desulfovibrio vulgaris Miyazaki F | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified preparation is brwon in color | Desulfovibrio vulgaris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 141 | - |
pH 7.5, temperature not specified in the publication | Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | when coupled with hydrogenase and methyl viologen, the enzyme stoichiometrically reduces thiosulfate to sulfite and sulfide with consumption of hydrogen. No substrates: sulfite or trithionate | Desulfovibrio vulgaris | ? | - |
? | |
| additional information | when coupled with hydrogenase and methyl viologen, the enzyme stoichiometrically reduces thiosulfate to sulfite and sulfide with consumption of hydrogen. No substrates: sulfite or trithionate | Desulfovibrio vulgaris Miyazaki F | ? | - |
? | |
| thiosulfate + 2 ferrocytochrome c3 | - |
Desulfovibrio vulgaris | sulfite + hydrogen sulfide + 2 ferricytochrome c3 | - |
? | |
| thiosulfate + 2 ferrocytochrome c3 | - |
Desulfovibrio vulgaris Miyazaki F | sulfite + hydrogen sulfide + 2 ferricytochrome c3 | - |
? | |
| thiosulfate + reduced methyl viologen | - |
Desulfovibrio vulgaris | sulfite + hydrogen sulfide + oxidized methyl viologen | - |
? | |
| thiosulfate + reduced methyl viologen | - |
Desulfovibrio vulgaris Miyazaki F | sulfite + hydrogen sulfide + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE | Desulfovibrio vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.9 | - |
Desulfovibrio vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c3 | - |
Desulfovibrio vulgaris |
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