Cloned (Comment) | Organism |
---|---|
recombinant expression of Strep II-tagged enzyme | Allochromatium vinosum |
Protein Variants | Comment | Organism |
---|---|---|
C138H | inactive | Campylobacter jejuni |
C138M | inactive | Campylobacter jejuni |
N254K | the enzyme shows 10fold reduced oxidation activity of thiosulfate but has a comparable maximum rate of tetrathionate reduction compared to the wild type enzyme | Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the enzyme bound hemes | Allochromatium vinosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum | - |
tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Campylobacter jejuni | - |
tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum | cofactor with a heme c | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum DSM 180 | cofactor with a heme c | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum NCIMB 10441 | cofactor with a heme c | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum ATCC 17899 | cofactor with a heme c | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum NBRC 103801 | cofactor with a heme c | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | Allochromatium vinosum D | cofactor with a heme c | tetrathionate + 2 ferrocytochrome c | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allochromatium vinosum | - |
- |
- |
Allochromatium vinosum | D3RVD4 | - |
- |
Allochromatium vinosum ATCC 17899 | D3RVD4 | - |
- |
Allochromatium vinosum D | D3RVD4 | - |
- |
Allochromatium vinosum DSM 180 | D3RVD4 | - |
- |
Allochromatium vinosum NBRC 103801 | D3RVD4 | - |
- |
Allochromatium vinosum NCIMB 10441 | D3RVD4 | - |
- |
Campylobacter jejuni | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 thiosulfate + 2 ferricyanide | - |
Campylobacter jejuni | tetrathionate + 2 ferrocyanide | - |
? | |
2 thiosulfate + 2 ferricyanide | highest activity | Allochromatium vinosum | tetrathionate + 2 ferrocyanide | - |
? | |
2 thiosulfate + 2 ferricytochrome c | - |
Allochromatium vinosum | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | - |
Campylobacter jejuni | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | cofactor with a heme c | Allochromatium vinosum | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | for AvTsdA the maximum rate of oxidative catalysis greatly exceeds that of reductive catalysis, cofactor with a heme c | Allochromatium vinosum | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | cofactor with a heme c | Allochromatium vinosum DSM 180 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | for AvTsdA the maximum rate of oxidative catalysis greatly exceeds that of reductive catalysis, cofactor with a heme c | Allochromatium vinosum DSM 180 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | cofactor with a heme c | Allochromatium vinosum NCIMB 10441 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | for AvTsdA the maximum rate of oxidative catalysis greatly exceeds that of reductive catalysis, cofactor with a heme c | Allochromatium vinosum NCIMB 10441 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | cofactor with a heme c | Allochromatium vinosum ATCC 17899 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | for AvTsdA the maximum rate of oxidative catalysis greatly exceeds that of reductive catalysis, cofactor with a heme c | Allochromatium vinosum ATCC 17899 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | cofactor with a heme c | Allochromatium vinosum NBRC 103801 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | for AvTsdA the maximum rate of oxidative catalysis greatly exceeds that of reductive catalysis, cofactor with a heme c | Allochromatium vinosum NBRC 103801 | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | cofactor with a heme c | Allochromatium vinosum D | tetrathionate + 2 ferrocytochrome c | - |
r | |
2 thiosulfate + 2 ferricytochrome c | for AvTsdA the maximum rate of oxidative catalysis greatly exceeds that of reductive catalysis, cofactor with a heme c | Allochromatium vinosum D | tetrathionate + 2 ferrocytochrome c | - |
r | |
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Allochromatium vinosum | ? | - |
- |
|
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Allochromatium vinosum DSM 180 | ? | - |
- |
|
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Allochromatium vinosum NCIMB 10441 | ? | - |
- |
|
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Allochromatium vinosum ATCC 17899 | ? | - |
- |
|
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Allochromatium vinosum NBRC 103801 | ? | - |
- |
|
additional information | the enzyme functions as a tetrathionate reductase and a thiosulfate oxidase, respectively | Allochromatium vinosum D | ? | - |
- |
|
tetrathionate + reduced methyl viologen | highest activity | Campylobacter jejuni | 2 thiosulfate + methyl viologen | - |
? | |
tetrathionate + reduced methyl viologen | very low activity | Allochromatium vinosum | 2 thiosulfate + methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28142, calculated from amino acid sequence | Allochromatium vinosum |
? | x * 28142, mature protein with two c-hemes, without signal peptide, followed by a C-terminal extension of one additional amino acid and the Strep II-tag, sequence calculation | Allochromatium vinosum |
? | x * 37103, calculated from amino acid sequence | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
AvTsdA | - |
Allochromatium vinosum |
di-heme TsdA | - |
Allochromatium vinosum |
tetrathionate reductase | - |
Campylobacter jejuni |
thiosulfate dehydrogenase | - |
Allochromatium vinosum |
thiosulfate oxidase | - |
Allochromatium vinosum |
TsdA | - |
Allochromatium vinosum |
TsdA | - |
Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Allochromatium vinosum | |
cytochrome c | - |
Campylobacter jejuni | |
heme | - |
Allochromatium vinosum | |
heme | - |
Campylobacter jejuni | |
heme | two heme c, heme 1 and heme 2, di-Fe(III), rapid interheme electron transfer determined from the AvTsdA crystal structure. Distal ligation of AvTsdA heme 2 is provided by Lys208 and Met209 | Allochromatium vinosum |
General Information | Comment | Organism |
---|---|---|
physiological function | thiosulfate dehydrogenases (TsdAs) are bidirectional bacterial di-heme enzymes that catalyze the interconversion of tetrathionate and thiosulfate at measurable rates in both directions, cf. EC 1.8.2.2, thiosulfate dehydrogenase. The active site heme 1 in the enzyme has His/Cys ligation in the ferric and ferrous states In Allochromatium vinosum TsdA, heme 2 reduction triggers a switch from His/Lys ligation to His/Met, but the rates of interconversion are such that His/Lys ligation is retained during turnover. Thiosulfate oxidation by enzyme AvTsdA provides electrons for photosynthesis | Allochromatium vinosum |