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Literature summary for 1.8.2.4 extracted from

  • McDevitt, C.; Hugenholtz, P.; Hanson, G.; McEwan, A.
    Molecular analysis of dimethyl sulphide dehydrogenase from Rhodovulum sulfidophilum: Its place in the dimethyl sulphoxide reductase family of microbial molybdopterin-containing enzymes (2002), Mol. Microbiol., 44, 1575-1587.
    View publication on PubMed
Search for more literature for 1.8.2.4

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Rhodovulum sulfidophilum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains 17 mol iron per mol enzyme Rhodovulum sulfidophilum
Molybdenum the enzyme contains 0.5 mol molybdenum per mol enzyme Rhodovulum sulfidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 subunit DdhC Rhodovulum sulfidophilum
32000
-
 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum
38000
-
 subunit DdhB Rhodovulum sulfidophilum
38000
-
 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum
94000
-
 subunit DdhA Rhodovulum sulfidophilum
94000
-
 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethyl sulfide + ferricytochrome c2 + H2O Rhodovulum sulfidophilum
-
 dimethyl sulfoxide + ferrocytochrome c2
-
 ?
dimethyl sulfide + ferricytochrome c2 + H2O Rhodovulum sulfidophilum SH1
-
 dimethyl sulfoxide + ferrocytochrome c2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodovulum sulfidophilum Q8GPG1 subunit gamma (DdhC)
-
Rhodovulum sulfidophilum Q8GPG3 subunit beta (DdhB)
-
Rhodovulum sulfidophilum Q8GPG4 subunit alpha (DdhA)
-
Rhodovulum sulfidophilum SH1 Q8GPG1 subunit gamma (DdhC)
-
Rhodovulum sulfidophilum SH1 Q8GPG3 subunit beta (DdhB)
-
Rhodovulum sulfidophilum SH1 Q8GPG4 subunit alpha (DdhA)
-

Purification (Commentary)

Purification (Comment) Organism
Poros 20HQ Strong anion exchange column chromatography and gel filtration Rhodovulum sulfidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethyl sulfide + ferricytochrome c2 + H2O
-
 Rhodovulum sulfidophilum dimethyl sulfoxide + ferrocytochrome c2
-
 ?
dimethyl sulfide + ferricytochrome c2 + H2O
-
 Rhodovulum sulfidophilum SH1 dimethyl sulfoxide + ferrocytochrome c2
-
 ?
dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Rhodovulum sulfidophilum dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
 ?
dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Rhodovulum sulfidophilum SH1 dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum

Synonyms

Synonyms Comment Organism
DdhA subunit Rhodovulum sulfidophilum
DdhB subunit Rhodovulum sulfidophilum
DdhC subunit Rhodovulum sulfidophilum
dimethyl sulphide dehydrogenase
-
 Rhodovulum sulfidophilum
DMS dehydrogenase
-
 Rhodovulum sulfidophilum
DMS DH
-
 Rhodovulum sulfidophilum

Cofactor

Cofactor Comment Organism Structure
molybdopterin bis(molybdopterin guanine dinucleotide)Mo Rhodovulum sulfidophilum
[3Fe-4S]-center subunit DdhB binds one 3Fe-4S cluster Rhodovulum sulfidophilum

General Information

General Information Comment Organism
malfunction the ddhA mutant is not able to grow photoautotrophically with dimethyl sulfide as electron donor, although photoheterotrophic growth is not impaired Rhodovulum sulfidophilum
physiological function dimethyl sulfide dehydrogenase is essential for photolithotrophic growth of Rhodovulum sulfidophilum on dimethyl sulfide but not for chemo-trophic growth on the same substrate Rhodovulum sulfidophilum