BRENDA - Enzyme Database
show all sequences of 1.8.2.5

Purification and properties of thiosulfate reductase from Desulfovibrio gigas

Hatchikian, E.; Arch. Microbiol. 105, 249-256 (1975) View publication on PubMed

Data extracted from this reference:

General Stability
General Stability
Organism
enzyme loses activtiy upon freezing and thawing
Desulfovibrio gigas
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
0.02 mM, 100% inhibition. Inhibition can be partially or completely reversed by cysteine
Desulfovibrio gigas
Ag+
0.01 mM, 90% inhibition
Desulfovibrio gigas
Hg2+
0.01 mM, 100% inhibition
Desulfovibrio gigas
iodoacetate
0.5 mM, 70% inhibition
Desulfovibrio gigas
N-ethylmaleimide
0.5 mM, 55% inhibition
Desulfovibrio gigas
o-Iodosobenzoate
0.05 mM, 52% inhibition
Desulfovibrio gigas
sulfite
3 mM, 45% inhibition
Desulfovibrio gigas
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
thiosulfate
37°C, pH 7.6
Desulfovibrio gigas
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
220000
-
gel filtration
Desulfovibrio gigas
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thiosulfate + 2 ferrocytochrome c3
Desulfovibrio gigas
-
sulfite + hydrogen sulfide + 2 ferricytochrome c3
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Desulfovibrio gigas
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
unstable during purification
Desulfovibrio gigas
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
41
-
37°C, pH 7.6
Desulfovibrio gigas
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
enzyme catalyzes the stoichiometric production of hydrogen sulfide and sulfite from thiosulfate. The most efficient coupling is obtained with a system containing cytochromes c3. The enzyme exhibits tetrathionate reductase activity. It does not show sulfite reductase activity
739959
Desulfovibrio gigas
?
-
-
-
?
thiosulfate + 2 ferrocytochrome c3
-
739959
Desulfovibrio gigas
sulfite + hydrogen sulfide + 2 ferricytochrome c3
-
-
-
?
thiosulfate + reduced methyl viologen
-
739959
Desulfovibrio gigas
sulfite + hydrogen sulfide + oxidized methyl viologen
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8
-
Desulfovibrio gigas
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8.8
-
8% of maximum activity
Desulfovibrio gigas
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c3
-
Desulfovibrio gigas
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c3
-
Desulfovibrio gigas
General Stability (protein specific)
General Stability
Organism
enzyme loses activtiy upon freezing and thawing
Desulfovibrio gigas
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
0.02 mM, 100% inhibition. Inhibition can be partially or completely reversed by cysteine
Desulfovibrio gigas
Ag+
0.01 mM, 90% inhibition
Desulfovibrio gigas
Hg2+
0.01 mM, 100% inhibition
Desulfovibrio gigas
iodoacetate
0.5 mM, 70% inhibition
Desulfovibrio gigas
N-ethylmaleimide
0.5 mM, 55% inhibition
Desulfovibrio gigas
o-Iodosobenzoate
0.05 mM, 52% inhibition
Desulfovibrio gigas
sulfite
3 mM, 45% inhibition
Desulfovibrio gigas
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
thiosulfate
37°C, pH 7.6
Desulfovibrio gigas
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
220000
-
gel filtration
Desulfovibrio gigas
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thiosulfate + 2 ferrocytochrome c3
Desulfovibrio gigas
-
sulfite + hydrogen sulfide + 2 ferricytochrome c3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
unstable during purification
Desulfovibrio gigas
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
41
-
37°C, pH 7.6
Desulfovibrio gigas
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
enzyme catalyzes the stoichiometric production of hydrogen sulfide and sulfite from thiosulfate. The most efficient coupling is obtained with a system containing cytochromes c3. The enzyme exhibits tetrathionate reductase activity. It does not show sulfite reductase activity
739959
Desulfovibrio gigas
?
-
-
-
?
thiosulfate + 2 ferrocytochrome c3
-
739959
Desulfovibrio gigas
sulfite + hydrogen sulfide + 2 ferricytochrome c3
-
-
-
?
thiosulfate + reduced methyl viologen
-
739959
Desulfovibrio gigas
sulfite + hydrogen sulfide + oxidized methyl viologen
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8
-
Desulfovibrio gigas
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8.8
-
8% of maximum activity
Desulfovibrio gigas
Other publictions for EC 1.8.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740755
Kurth
Electron accepting units of th ...
Marichromatium purpuratum, Marichromatium purpuratum 984
J. Biol. Chem.
291
24804-24818
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739959
Hatchikian
Purification and properties of ...
Desulfovibrio gigas
Arch. Microbiol.
105
249-256
1975
-
-
-
-
-
1
7
1
-
-
1
1
-
1
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
7
-
1
-
-
1
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
740591
Haschke
Thiosulfate reductase of Desul ...
Desulfovibrio vulgaris
J. Bacteriol.
106
603-607
1971
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
1
1
-
-
-
-
-
3
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
740631
Nakatsukasa
Thiosulfate reductase isolated ...
Desulfotomaculum nigrificans
J. Bacteriol.
98
429-433
1969
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
2
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
740639
Ishimoto
-
Biochemical studies on sulfate ...
uncultured bacterium
J. Biochem.
44
233-242
1957
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-