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Literature summary for 1.8.3.1 extracted from
- The G473D mutation impairs dimerization and catalysis in human sulfite oxidase (2006), Biochemistry, 45, 2149-2160.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain TP1000 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G473A | mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type | Homo sapiens |
| G473D | monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation | Homo sapiens |
| G473W | monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation | Homo sapiens |
| R212A/G473D | mutant is able to oligomerize but has undetectable activity, significant random-coil formation | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00043 | - |
cytochrome c | mutant enzyme G473A, pH 8.5 | Homo sapiens |  |
| 0.00082 | - |
cytochrome c | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
| 0.00123 | - |
cytochrome c | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
| 0.00129 | - |
sulfite | wild type enzyme, pH 6.0 | Homo sapiens | |
| 0.00141 | - |
cytochrome c | wild type enzyme, pH 7.0 | Homo sapiens | |
| 0.00162 | - |
sulfite | wild type enzyme, pH 6.5 | Homo sapiens | |
| 0.00173 | - |
cytochrome c | wild type enzyme, pH 6.0 | Homo sapiens | |
| 0.00272 | - |
sulfite | wild type enzyme, pH 7.0 | Homo sapiens | |
| 0.00339 | - |
sulfite | wild type enzyme, pH 7.5 | Homo sapiens | |
| 0.00371 | - |
cytochrome c | mutant enzyme G473D, pH 8.6 | Homo sapiens | |
| 0.00435 | - |
sulfite | wild type enzyme, pH 8.0 | Homo sapiens | |
| 0.0044 | - |
cytochrome c | wild type enzyme, pH 8.5 | Homo sapiens | |
| 0.00453 | - |
sulfite | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
| 0.00474 | - |
cytochrome c | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
| 0.00536 | - |
sulfite | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
| 0.00536 | - |
cytochrome c | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
| 0.00825 | - |
sulfite | wild type enzyme, pH 8.5 | Homo sapiens | |
| 0.014 | - |
cytochrome c | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
| 0.0172 | - |
sulfite | mutant enzyme G473A, pH 7.4 | Homo sapiens | |
| 0.0221 | - |
sulfite | wild type enzyme, pH 9.0 | Homo sapiens | |
| 0.0357 | - |
cytochrome c | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
| 0.0362 | - |
cytochrome c | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
| 0.0487 | - |
sulfite | mutant enzyme G473A, pH 8.0 | Homo sapiens | |
| 0.0635 | - |
cytochrome c | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
| 0.0671 | - |
sulfite | wild type enzyme, pH 9.5 | Homo sapiens | |
| 0.0905 | - |
cytochrome c | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
| 0.107 | - |
sulfite | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
| 0.107 | - |
cytochrome c | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
| 0.33 | - |
sulfite | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
| 0.623 | - |
sulfite | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
| 0.774 | - |
sulfite | mutant enzyme G473A, pH 9.1 | Homo sapiens | |
| 0.99 | - |
sulfite | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
| 1.063 | - |
sulfite | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
| 1.223 | - |
sulfite | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
| 1.66 | - |
sulfite | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
| 1.91 | - |
sulfite | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
| 2.034 | - |
sulfite | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
| 2.04 | - |
sulfite | mutant enzyme G473D, pH 8.5 | Homo sapiens | |
| 3.684 | - |
sulfite | mutant enzyme G473A, pH 10.0 | Homo sapiens | |
| 10.41 | - |
sulfite | mutant enzyme G473W, pH 9.0 | Homo sapiens | |
| 25.88 | - |
sulfite | mutant enzyme G473D, pH 9.1 | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
| 52000 | - |
2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
| 53500 | - |
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
| 54500 | - |
1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
| 66600 | - |
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
| 101000 | - |
wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl-Sepharose column chromatography and Superdex 200 16/60 FPLC column gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + cytochrome c | - |
Homo sapiens | sulfate + reduced cytochrome c | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
| monomer | 1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
| monomer | 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
| monomer | 1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
| monomer | 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SO | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
sulfite | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
| 0.28 | - |
sulfite | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
| 0.34 | - |
cytochrome c | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
| 0.42 | - |
sulfite | mutant enzyme G473D, pH 9.1 | Homo sapiens | |
| 0.48 | - |
cytochrome c | mutant enzyme G473D, pH 8.6 | Homo sapiens | |
| 0.5 | - |
sulfite | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
| 0.53 | - |
cytochrome c | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
| 0.54 | - |
sulfite | mutant enzyme G473D, pH 8.5 | Homo sapiens | |
| 0.57 | - |
sulfite | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
| 0.58 | - |
sulfite | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
| 0.59 | - |
cytochrome c | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
| 0.6 | - |
sulfite | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
| 0.62 | - |
cytochrome c | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
| 0.78 | - |
cytochrome c | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
| 1.35 | - |
sulfite | mutant enzyme G473W, pH 9.0 | Homo sapiens | |
| 1.67 | - |
cytochrome c | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
| 1.8 | - |
sulfite | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
| 1.95 | - |
cytochrome c | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
| 2.48 | - |
sulfite | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
| 2.94 | - |
cytochrome c | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
| 3.83 | - |
cytochrome c | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
| 4.15 | - |
sulfite | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
| 12.4 | - |
cytochrome c | wild type enzyme, pH 6.0 | Homo sapiens | |
| 12.8 | - |
cytochrome c | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
| 13.2 | - |
sulfite | wild type enzyme, pH 6.0 | Homo sapiens | |
| 15.1 | - |
sulfite | mutant enzyme G473A, pH 10.0 | Homo sapiens | |
| 15.9 | - |
sulfite | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
| 17.7 | - |
sulfite | wild type enzyme, pH 6.5 | Homo sapiens | |
| 18.1 | - |
cytochrome c | wild type enzyme, pH 7.0 | Homo sapiens | |
| 21.6 | - |
sulfite | mutant enzyme G473A, pH 7.4 | Homo sapiens | |
| 24.2 | - |
sulfite | wild type enzyme, pH 7.0 | Homo sapiens | |
| 24.7 | - |
sulfite | wild type enzyme, pH 7.5 | Homo sapiens | |
| 25.4 | - |
cytochrome c | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
| 25.7 | - |
sulfite | wild type enzyme, pH 9.0 | Homo sapiens | |
| 25.9 | - |
sulfite | wild type enzyme, pH 8.0 | Homo sapiens | |
| 26.2 | - |
sulfite | mutant enzyme G473A, pH 8.0 | Homo sapiens | |
| 26.3 | - |
sulfite | wild type enzyme, pH 9.5 | Homo sapiens | |
| 26.9 | - |
cytochrome c | wild type enzyme, pH 8.5 | Homo sapiens | |
| 26.9 | - |
sulfite | wild type enzyme, pH 8.5 | Homo sapiens | |
| 28.4 | - |
sulfite | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
| 31.9 | - |
sulfite | mutant enzyme G473A, pH 9.1 | Homo sapiens | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Homo sapiens |
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