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Literature summary for 1.8.3.1 extracted from

  • Bender, D.; Tobias Kaczmarek, A.; Niks, D.; Hille, R.; Schwarz, G.
    Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase (2019), Biochem. J., 476, 1805-1815 .
    View publication on PubMed
Search for more literature for 1.8.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TP1004 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
nitrite nitrite inhibits sulfite-dependent cytochrome c reduction at sulfite concentrations ranging from 0.01 to 0.1 mM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion mitochondrial intermembrane space Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + O2 + H2O Homo sapiens
-
 sulfate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P51687
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + H2O + porcine ferricytochrome c the nitrite reduction mechanism involves sulfite oxidation, sulfate release and nitrite coordination at molybdenum with protonation-dependent nitric oxide and molybdenum V release. The highest nitric oxide production occurs between 0.01 and 0.0375 mM sulfite, with a dose-dependent inhibition of nitric oxide formation at higher sulfite concentrations Homo sapiens nitric oxide + porcine ferrocytochrome c
-
 ?
sulfite + H2O + porcine ferricyanide
-
 Homo sapiens sulfate + porcine ferrocyanide
-
 ?
sulfite + H2O + porcine ferricytochrome c
-
 Homo sapiens sulfate + porcine ferrocytochrome c
-
 ?
sulfite + O2 + H2O
-
 Homo sapiens sulfate + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens
molybdenum cofactor
-
 Homo sapiens