Any feedback?
Literature summary for 1.8.3.2 extracted from
- Sulfhydryl oxidase from rat skin (1987), Methods Enzymol., 143, 510-515.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | at high concentrations | Rattus norvegicus |  |
| iodoacetamide | - |
Rattus norvegicus | |
| iodoacetic acid | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | native activity is increased 2fold | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skin | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-mercaptoethanol + O2 | 3.7% of the activity with dithiothreitol | Rattus norvegicus | ? + H2O | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + O2 | - |
Rattus norvegicus | ? + H2O | - |
? | |
| D-penicillamine + O2 | 33% of the activity with dithiothreitol | Rattus norvegicus | ? + H2O | - |
? | |
| dithioerythritol + O2 | - |
Rattus norvegicus | ? + H2O | - |
? | |
| dithiothreitol + O2 | - |
Rattus norvegicus | ? + H2O | - |
? | |
| GSH + O2 + O2 | 0.7% of the activity with dithiothreitol | Rattus norvegicus | GSSG + H2O | - |
? | |
| L-Cys + O2 | 17% of the activity with dithiothreitol | Rattus norvegicus | ? + H2O | - |
? | |
| N-acetylcysteine + O2 | 4.6% of the activity with dithiothreitol | Rattus norvegicus | ? + H2O | - |
? | |
| reductively denatured ribonuclease A + O2 | - |
Rattus norvegicus | renatured ribonuclease + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dithiothreitol, 1 mM, increases the stability to heating | Rattus norvegicus |
| 60 | - |
15 min, 85% loss of activity. 30 min, complete loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.2 | oxidation of 5,5'-dithiobis(2-nitrobenzoic acid) and reactivation of ribonuclease | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
